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Toxoplasma gondii possesses three protein kinase A (PKA) orthologs. PKA C3 has been implicated as a negative regulator of transformation from the acute stage tachyzoites to the chronic stage bradyzoites. We performed immunoprecipitation of PKA C3 and identified interaction partners by mass spectrome...
ORGANISM(S): Toxoplasma gondii GT1 
2024-07-12 | PXD039896 | Pride
To broadly explore artemisinin susceptibility in apicomplexan parasites, we used genome-scale CRISPR screens recently developed for Toxoplasma gondii to discover sensitizing and desensitizing mutations. From these screens, we identified the mitochondrial protease DegP2, which appeared gDegP2-respons...
ORGANISM(S): Toxoplasma gondii GT1 
2020-08-28 | PXD019917 | Pride
reviously, we characterized a chemical modulator, ENH1, that exhibited potent antiparasitic properties against apicomplexans and induced calcium oscillations, but the target eluded identification. To determine the target of ENH1, we employed thermal proteome profiling in parasites. We treated extrac...
ORGANISM(S): Toxoplasma gondii GT1 
2023-08-21 | PXD018818 | Pride
Previously, we characterized a chemical modulator, ENH1, that exhibited potent antiparasitic properties against apicomplexans and induced calcium oscillations, but the target eluded identification. To determine the target of ENH1, we employed thermal proteome profiling in parasites. We treated extra...
ORGANISM(S): Toxoplasma gondii GT1 
2020-07-13 | PXD018810 | Pride
Identification of target transcripts for the putative chloroplast RNA binding protein CRP1 in Zea mays. CRP1 was immunoprecipitated from a chloroplast extract. Chloroplast extracts were prepared from WT and CRP1-deficient tissue. RNA from the pellet and from the supernatant for each pulldown was lab...
ORGANISM(S): Zea mays 
Immunoprecipitation of the chloroplast splicing factor CAF1 and analysis of bound RNAs with a complete Zea mays cpDNA microarray.
ORGANISM(S): Zea mays 
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