Sort   by:  
 Page size 
TranscriptomicsModulation of murine bone marrow derived dendritic cells by IL-4
The archetypal Th2 cytokine interleukin 4 (IL-4) has previously been shown to alternatively activate dendritic cells (DCs) both in vitro and in vivo.To more fully understand the impact of IL-4 dependent alternative activation of DCs, we used mRNA microarrays to define their transcriptional profile. ...
ORGANISM(S): Mus musculus 
2015-04-28 | E-GEOD-59868 | biostudies-arrayexpress
TranscriptomicsSORT-seq of Synovial Tissue Dentritic Cells from Rheumatoid arthritis
SORT-seq is a plate-based method of single-cell RNA sequencing (Muraro et al. 2016) is a partially robotized version of the CEL-seq2 protocol (Hashimshony et al. 2016). Synovial membranes from Rheumatoid arthritis' patients yield a relatively robust number of DCs using scRNAseq, we sought to valida...
ORGANISM(S): Homo sapiens 
2024-12-09 | E-MTAB-14198 | biostudies-arrayexpress
TranscriptomicsSingle cell RNA and CITE sequencing of cells from synovial tissue biopsies of healthy donors, and Rheumatoid Arthritis (RA) patients with active disease or sustained clinical remission
The dataset includes the raw FASTQ files generated from the 10x Genomics Single Cell 3’ v3.1 experiments. For samples processed with TotalSeq Hashtag reagents, the corresponding hashtag oligonucleotide and condition annotations are provided as separate .csv files. The processed matrices contain filt...
ORGANISM(S): Homo sapiens 
2024-12-06 | E-MTAB-14213 | biostudies-arrayexpress
TranscriptomicsCo-culture of synovial tissue dendritic cells with autologous peripheral blood memory T-Cells across Active and Remission RA
To dissect the precise roles of ST-DC2 clusters and ST-iDC3 clusters in T-cell activation, we co-cultured each cluster separately with autologous memory CD4pos T-cells. FACS-sorted ST-DC2 clusters and ST-iDC3 clusters from synovial tissue of active RA or remission RA were co-cultured for 5 days wit...
ORGANISM(S): Homo sapiens 
2024-12-09 | E-MTAB-14191 | biostudies-arrayexpress
TranscriptomicsEvaluation of transcriptomic profiles of peripheral blood myeloid cell subsets of RA patients in remission prior to cDMARD withdrawal, and at the point of flare / 6 months after in sustained remission
To test the contribution of distinct ST-DC populations to either autoimmunity and inflammation or sustained disease remission, we evaluated the phenotypes of their blood precursors in a human model of disease flare following treatment withdrawal in RA patients in disease remission (the BioRRA study,...
ORGANISM(S): Homo sapiens 
2024-12-09 | E-MTAB-14169 | biostudies-arrayexpress
TranscriptomicsSingle cell RNA sequencing of paired PBMC and synovial tissue cells from Rheumatoid Arthritis (RA) patients naive to treatment
Live CD45+ cells from the peripheral blood (PB) and synovial tissue (ST) of 3 naive to treatment RA patients were sorted for single cell RNA sequencing (scRNAseq) using 10x Genomics platform. Library preparation was performed using 10X Single Cell 3’ Reagent Kits v2 and libraries were sequenced on t...
ORGANISM(S): Homo sapiens 
2024-12-09 | E-MTAB-14192 | biostudies-arrayexpress
TranscriptomicsA dominant role for the methyl-CpG-binding protein Mbd2 in controlling Th2 induction by dendritic cells [gene expression]
Dendritic cells (DCs) direct CD4+ T cell differentiation into distinct T helper (Th) subsets that are vital for protection against diverse types of infection. However, the mechanisms employed by DCs to initiate Th2 responses, which are important for immunity to helminth infection as well as being a...
ORGANISM(S): Mus musculus 
2015-04-20 | E-GEOD-66096 | biostudies-arrayexpress
TranscriptomicsSingle-Cell RNA Sequencing of Mouse Synovial Tissue Across Developmental Time Points
Synovial joints harbor a protective lining layer, consisting of fibroblasts and macrophages, which form an epithelial-like barrier. In inflamed joints, lining macrophages regulate both early inflammatory cell influx and resolution. Despite these critical functions, it is currently unknown at what st...
ORGANISM(S): Mus musculus 
2026-06-09 | E-MTAB-17170 | biostudies-arrayexpress
GenomicsA dominant role for the methyl-CpG-binding protein Mbd2 in controlling Th2 induction by dendritic cells [ChIP-Seq]
Dendritic cells (DCs) direct CD4+ T cell differentiation into distinct T helper (Th) subsets that are vital for protection against diverse types of infection. However, the mechanisms employed by DCs to initiate Th2 responses, which are important for immunity to helminth infection as well as being a...
ORGANISM(S): Mus musculus 
2015-04-20 | E-GEOD-66198 | biostudies-arrayexpress
TranscriptomicsLai_CMEW1_Cl_8
modENCODE_submission_2721 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their re...
ORGANISM(S): Drosophila melanogaster 
2010-10-18 | E-GEOD-24307 | biostudies-arrayexpress
Sort   by:  
 Page size