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Myeloid Angiogenic Cells (MACs) were infected with the intracellular, bacterial pathogen Bartonella henselae (B.h.). Infected cells were seeded onto Matrigel coated plates. While uninfected cells showed no phenotypic changes and died over time, infected cells showed strong phenotypic changes and dev...
ORGANISM(S): Homo sapiens 
Background Proteomic characterization of microglia has been limited by low yield and contamination by non-microglial proteins by magnetic-activated cell sorting (MACS) enrichment strategies. To determine whether a fluorescent-activated cell sorting (FACS)-based strategy overcomes these limitations,...
ORGANISM(S): Mus musculus (Mouse) 
2020-05-11 | PXD015652 | Pride
effect of different T cell activations (CD3 and CD3-CD28) prior retroviral transduction and different selection procedures (G418 or NGFR-MACS)
ORGANISM(S): Homo sapiens 
to investigate the RA regulated genes in 2 dpp thy1+ gonocytes Experiment Overall Design: gonocytes were isolated from 2 dpp mouse testes by THY1+ MACS sorting and cultured without serum, growth factors and feeder cells for 8hr, with either vehicle ethanol or RA (100 nM). Two control and two RA trea...
ORGANISM(S): Mus musculus 
Luminal, basal, stromal, and endothelial cells were MACS sorted from whole tissue. Targets from five biological replicates of each were generated and the expression profiles were determined using Affymetrix U133 Plus 2.0 arrays. These data represent cell specific transcriptomes.
ORGANISM(S): Homo sapiens 
Comparison of the DNA methylation profiles of CD14+ monocytes from human peripheral blood with derived dendritic cells (DCs) and macrophages (MACs) obtained by exposure with GM-CSF/IL-4 and GM-CSF, respectively, and with mature DCs and MACs after lipopolysaccharide (LPS) exposure The methylation pro...
ORGANISM(S): Homo sapiens 
We are interested in unveiling potential gene expression alterations underlying the T cell-related phenotypes observed in DUSP4-/- mice. We used microarray to obtain a macroscopic views of gene profiles comparing freshly isolated splenic T cells from WT and DUSP4-/- mice Magnetic-assisted cell sorti...
ORGANISM(S): Mus musculus 
Age-related decline in brain endothelial cell (BEC) function critically contributes to cerebrovascular and neurodegenerative disease. Comprehensive atlases of the BEC transcriptome have become available but results from proteomic profiling are lacking. To gain insights into endothelial pathways affe...
ORGANISM(S): Mus musculus (Mouse) 
2024-02-15 | PXD044993 | Pride
The objective of the study was to identify differentially methylated regions of DNA (DMRs) that distinguish human leukocyte subtypes, and hence serve as biomarkers for those immune cell types. This file contains Illumina Infinium HumanMethylation27 BeadChip data for human leukocyte subtypes that wer...
ORGANISM(S): Homo sapiens 
Multiple myeloma is a plasma cell malignancy of the bone marrow. Despite therapeutic advances, multiple myeloma remains incurable and better risk stratification as well as new therapies are therefore highly needed. The proteome of multiple myeloma has not been systematically assessed before and hold...
ORGANISM(S): Homo sapiens (Human) 
2024-07-09 | PXD043580 | Pride
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