We performed label-free liquid chromatography MS/MS spectrometry analysis of urine samples from 26 HUA patients and 25 healthy controls, attempting to establish the possible protein links between hyperuricemia and these diseases by profiling urine proteome.
APEX1 overexpression and knockdown cell lines were established based on SKOV3 cell line. Lable-free quantitative phosphoproteomics was done to evaluate the impact of APEX1 on cellular phosphoproteomics