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TranscriptomicsExpression Profiling of MAP Kinase-Mediated Meiotic Progression in C. elegans
A unique allele of MAP kinase, mpk-1(ga111), displays a reversible, temperature-sensitive, tissue-specific defect in progression through meiotic prophase I. We performed gene expression profiling on mpk-1(ga111) animals at multiple times after shift to permissive temperature to identify candidate d...
ORGANISM(S): Caenorhabditis elegans 
2010-07-01 | E-GEOD-5069 | biostudies-arrayexpress
MetabolomicsCombined transcriptome and metabolome analysis reveals the regulatory network of histidine kinase QseC in the two-component system of Glaesserella parasuis
Glaesserella parasuis is the causative agent of Glässer's disease in pigs, which can lead to polyserositis, arthritis, and meningitis. This study used transcriptome and metabolomics sequencing techniques to investigate the mechanism of action of qseC gene in Glaesserella parasuis. Transmission elect...
2025-08-11 | MTBLS12504 | MetaboLights
TranscriptomicsTranscription profiling of Arabidopsis reveals Flg22 regulates MAP kinase 6 interaction with an ethylene response factor substrate via ethylene
Despite their importance, plant MAP kinase targets are still poorly elucidated. Here, the specific in vivo interaction of an ethylene response factor (ERF104) with the Arabidopsis MAP kinase, MPK6, is shown by fluorescence resonance energy transfer. The interaction, which is lost within minutes afte...
ORGANISM(S): Arabidopsis thaliana 
2009-05-27 | E-GEOD-11807 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Arabdiopsis plants over-expressing MKK3DD or MKK4DD to identify genes acting downstream of MAP kinase kinase
To identify genes acting downstream of MAP kinase kinase, and thus map its signal, we used the steroid-inducible overexpression system. The constitutive active form MKK3 (MKK3DD) or MKK4 (MKK4DD) was cloned into the glucocorticoid-inducible vector pTA7002 and transformed using Agrobacterium method i...
ORGANISM(S): Arabidopsis thaliana 
2007-05-11 | E-MEXP-827 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of adult mouse heart following activation of Ras, MKK3 and MKK7 mutant genes
Three major MAP kinase signaling cascades, ERK, p38 and JNK, play significant roles in the development of cardiac hypertrophy and heart failure in response to external stress and neural/hormonal stimuli. In order to study the specific function of each MAP kinase branch in adult heart, we have gener...
ORGANISM(S): Mus musculus 
2007-10-12 | E-GEOD-3530 | biostudies-arrayexpress
TranscriptomicsDepletion of Erk1 and Erk2 MAP kinases in primary human keratinocytes
We assessed the effect of RNAi-mediated MAP kinase cascade signaling blockade in primary human keratinocytes. Two sets of siRNA targeting different regions of the Erk1/2 genes were used, enabling identification of off-target siRNA effects. Primary human keratinocytes were electroporated with scra...
ORGANISM(S): Homo sapiens 
2009-05-03 | E-GEOD-15417 | biostudies-arrayexpress
TranscriptomicsAberrant ERK signaling causes resistance to EGFR kinase inhibitors
The clinical efficacy of EGFR kinase inhibitors is limited by the development of drug resistance. The irreversible EGFR kinase inhibitor WZ4002 is effective against the most common mechanism of drug resistance mediated by the EGFR T790M mutation. Here we show that in multiple complementary models ha...
ORGANISM(S): Homo sapiens 
2012-10-01 | E-GEOD-37699 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of preimplantation mouse embryos to study the requirement of the MAP kinase signaling pathway for preimplantation development.
We examined expression profiles of genes that could be regulated by the MAPK pathways during mouse preimplantation development. We used Affymetrix GeneChips for microarray analysis, and three independent experiments were carried out. 8-cell stage embryos were treated or untreated with MAPK inhibit...
ORGANISM(S): Mus musculus 
2007-09-25 | E-GEOD-2229 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Arabidopsis mkk1, mkk2 and mkk1/2 knockout treated with the saliicylic acid analog BTH
Gene Expression profiling of the Arabidopsis thaliana MAP Kinase Kinases 1 (mkk1), MAP Kinase Kinases 2 (mkk2) knockout mutants and the double mutant mkk1/mkk2 before and 24 hours after treatment with the salicylic acid analog BTH, was measured by hybridisation to an Affymetrix ATH1 GeneChip. Experi...
ORGANISM(S): Arabidopsis thaliana 
2008-06-11 | E-GEOD-10646 | biostudies-arrayexpress
ProteomicsGadd45a Promotes Skeletal Muscle Atrophy by Forming a Complex with the Protein Kinase MEKK4
Skeletal muscle atrophy is a serious and highly prevalent condition that remains poorly understood at the molecular level. Previous work found that skeletal muscle atrophy involves an increase in skeletal muscle Gadd45a expression, which is necessary and sufficient for skeletal muscle fiber atrophy...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2016-05-17 | MSV000079730 | MassIVE
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