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ProteomicsQuantitative proteomic analysis reveals JMJD6 and DNAJB11 as endogenous substrates of E3 ligase RFFL
A549 cells were washed with ice-cold PBS three times and harvested using mechanical scraping. Cell pellets were processed as described previously (Marathe et al., 2021). Briefly, the pellet was re-solubilized in Urea-Thiourea Buffer (6M Urea, 2M Thio-urea in Tris-Cl) containing protease inhibitor co...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-01-15 | MSV000096861 | MassIVE
ProteomicsQuantitative proteomic analysis reveals JMJD6 and DNAJB11 as endogenous substrates of E3 ligase RFFL
Immunoprecipitation experiments using anti-RFFL antibody. Samples were loaded in technical duplicates in EvoTips, and chromatographic separation was done on an in-house column and the 30 samples per day (SPD) method on Evosep One system (Evosep). Peptides were detected and fragmented using timsTOF P...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-01-16 | MSV000096868 | MassIVE
ProteomicsLabel-free proteomics to study the effect of IL-9 on MDA-MB-231 and HeLa cells
To study the proteomic profile of HeLa and MDA-MB-231 upon IL-9 stimulation, we treated both the cell-lines with the cytokine for 24 hours. Quantitative label-free proteomic analysis was then performed and abundance ratios were obtained for proteins from cells in IL-9 treated v. untreated conditions...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-03-03 | MSV000086990 | MassIVE
ProteomicsDifferentially expressed proteins in Human Primary Keratinocytes upon IL-9 stimulation
HPKs from 3-5 healthy individual donors were pre-stimulated with TGFb (10ng/ml) plus IL-4 (20ng/ml) to upregulated the IL-9R (Das et al., 2019). Next day, HPKs were stimulated with IL-9 (50ng/ml) or in combination with IFNg (10ng/ml) for 24 hours at 37 degree C with 5% CO2. Sample preparation for L...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-01-28 | MSV000086776 | MassIVE
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