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ProteomicsAnalysis of FAM65A interactions
We identified FAM65A as a novel interactor of RHOA. To reveal other binding partners of FAM65A, we carried out a quantitative IP-MS, using SILAC labelled HeLa cells ectopically expressing GFP-FAM65A or GFP alone as control. Experiments were performed in duplicate with switched labelling and specific...
ORGANISM(S): Homo sapiens (Human) 
2016-11-01 | PXD004933 | Pride
Ip-ms  silac  fam65a  rho 
ProteomicsIdentification of novel RHOA interacting patners
RHO subfamily of small GTPases comprise highly conserved family members RHOA, RHOB, and RHOC which cycle between GTP-bound 'active' and GDP-bound 'inactive' states. In the active form, RHO proteins interact with a variety of downstream effector proteins, controlling their activity and function. Many...
ORGANISM(S): Homo sapiens (Human) 
2016-11-01 | PXD004934 | Pride
Silac  lc-ms  protein-protein interactions  rhoa 
Proteomics,MultiomicsProtrusion vs Cell-body SILAC proteomics
In mesenchymal-like cell migration, cells need to polarise into a protrusive front and a retracting cell body. To understand this process better, we set out to quantify the distribution of cellular proteins between protrusions and cell-body by proteomics, using MDA-MB-231 cells, a highly invasive br...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-03-10 | MSV000079570 | MassIVE
ProteomicsAnalysis of total proteome changes upon long-term treatment with Rho kinase inhibitor H1152.
We quantified protein changes associated with long-term Rho-kinase inhibition, using SILAC proteomics in conjugation with long-termH1152 treatment of mouse embryonic fibroblast cells.
ORGANISM(S): Mus musculus (Mouse) 
2016-01-18 | PXD002521 | Pride
Mouse embryonic fibroblasts  rock1/2  h1152  silac  cell cycle 
ProteomicsTemporal analysis of phospho-proteome changes following Rho-kinase inhibition.
To define short and long-term changes in the phospho-proteome of the cells following Rho-kinase inhibition with H1152 inhibitor, in mouse embryonic fibroblast cells.
ORGANISM(S): Mus musculus (Mouse) 
2016-01-18 | PXD002515 | Pride
Mouse embryonic fibroblasts  rock1/2  phospho-myosin light chain  h1152 
ProteomicsProtrusion vs Cell-body pulsed-SILAC proteomics
In mesenchymal-like cell migration, cells need to polarise into a protrusive front and a retracting cell body. Apart from proteins, several mRNAs are known to be localised to cell protrusions but to what extent RNA localisation and local translation contributes toward front-back assymetry is unknown...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2015-10-09 | PXD002649 | Pride
Pulsed-silac  local translation  protrusion 
ProteomicsProtrusion vs Cell-body SILAC proteomics
In mesenchymal-like cell migration, cells need to polarise into a protrusive front and a retracting cell body. To understand this process better, we set out to quantify the distribution of cellular proteins between protrusions and cell-body by proteomics, using MDA-MB-231 cells, a highly invasive br...
ORGANISM(S): Homo sapiens (Human) 
2015-10-09 | PXD000914 | Pride
Human silac proteomics protrusion lc-ms/ms orbitrap-velos maxquant 
TranscriptomicsPriming defense genes and metabolites in hybrid poplar by the green leaf volatile cis-3-hexenyl acetate
• Herbivore-induced plant volatiles (HIPVs), in addition to attracting natural enemies of herbivores, can serve a signaling function within plants by acting as wound signals that induce or prime defenses. However, particularly in woody plants, which compounds within HIPV blends are capable of act...
ORGANISM(S): Populus x canadensis 
2010-05-25 | E-GEOD-11955 | biostudies-arrayexpress
ProteomicsA Rac/Cdc42 exchange factor complex promotes formation of lateral filopodia...
3xFLAG-IPs from HEK293T cell lysates expressing 3xFLAG-bait or 3xFLAG-control. Trypsin Digestion
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2015-04-09 | MSV000079100 | MassIVE
UnknownOlfactory Dysfunction After SARS-CoV-2 Infection in the RECOVER Adult Cohort.
Not available
S-EPMC12464792 | biostudies-literature
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