Proteomics

Dataset Information

68

Analysis of total proteome changes upon long-term treatment with Rho kinase inhibitor H1152.


ABSTRACT: We quantified protein changes associated with long-term Rho-kinase inhibition, using SILAC proteomics in conjugation with long-termH1152 treatment of mouse embryonic fibroblast cells.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus musculus  

TISSUE(S): Embryo

DISEASE(S): Not Available

SUBMITTER: Sandra Kuemper  

PROVIDER: PXD002521 | Pride | 2016-01-18

REPOSITORIES: Pride

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Publications

Rho-associated kinase (ROCK) function is essential for cell cycle progression, senescence and tumorigenesis.

Kümper Sandra S   Mardakheh Faraz K FK   McCarthy Afshan A   Yeo Maggie M   Stamp Gordon W GW   Paul Angela A   Worboys Jonathan J   Sadok Amine A   Jørgensen Claus C   Guichard Sabrina S   Marshall Christopher J CJ  

eLife 20160114


Rho-associated kinases 1 and 2 (ROCK1/2) are Rho-GTPase effectors that control key aspects of the actin cytoskeleton, but their role in proliferation and cancer initiation or progression is not known. Here, we provide evidence that ROCK1 and ROCK2 act redundantly to maintain actomyosin contractility and cell proliferation and that their loss leads to cell-cycle arrest and cellular senescence. This phenotype arises from down-regulation of the essential cell-cycle proteins CyclinA, CKS1 and CDK1.  ...[more]

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