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Transcription profiling of wild type yeast strain as well as strains carrying a deletion of Gcn4, Arr1 or both. Gene expression in rich medium (YPD) and under osmotic stress conditions (YPD + 0.8M NaCl) was compared.
ORGANISM(S): Saccharomyces cerevisiae 
Yeast RNA polymerase (Pol) II consists of a ten-subunit core enzyme and the Rpb4/7 subcomplex, which is dispensable for catalytic activity and dissociates in vitro. To investigate whether Rpb4/7 is an integral part of DNA-associated Pol II in vivo, we used chromatin immuno-precipitation coupled to h...
ORGANISM(S): Saccharomyces cerevisiae 
To obtain rates of mRNA synthesis and decay in yeast, we established dynamic transcriptome analysis (DTA). DTA combines non-perturbing metabolic RNA labeling with dynamic kinetic modeling. DTA was used to monitor the cellular response to osmotic stress in comparison to the wild type. Genomic occupa...
ORGANISM(S): Saccharomyces cerevisiae 
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