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We tested the expression profile of 1146 miRNAs in PBMCs of 19 MS patients and 14 controls (discovery sample) and we explored their function either in vivo by performing a whole-genome mRNA profiling in same patients and in silico using bioinformatic prediction tool (miRbase software). These approac...
ORGANISM(S): Homo_sapiens 
We tested the expression profile of 1146 miRNAs in PBMCs of 19 MS patients and 14 controls (discovery sample) and we explored their function either in vivo by performing a whole-genome mRNA profiling in same patients and in silico using bioinformatic prediction tool (miRbase software). These approac...
ORGANISM(S): Homo_sapiens 
Essential Thrombocythemia (ET) patients, as well as normal donors BM CD34+ cells were differentiated in thrombopoietin-treated liquid suspension cultures. Briefly, CD34+ cells (80,000 /mL) were resuspended in a serum-free medium in the presence of 100 ng/mL of human recombinant thrombopoietin (TPO; ...
ORGANISM(S): Homo sapiens 
Normal donors BM CD34+ cells were differentiated in thrombopoietin-treated liquid suspension cultures. Briefly, CD34+ cells (80,000 /mL) were resuspended in a serum-free medium in the presence of 100 ng/mL of human recombinant thrombopoietin (TPO; Genzyme, Boston, MA). Every 3 days, viable cells wer...
ORGANISM(S): Homo sapiens 
To evaluate wether miRNA expression patterns contributes to obesity total RNA were purified from subcutaneous adipose tissue (SAT)and used in miRNA microarrays. Platform contain LNA-modified probes for all human miRNAs present in release 8.2 of the miRBase microRNA Registry. Two RNA pools from 3 n...
ORGANISM(S): Homo sapiens 
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