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TranscriptomicsRNA-Seq of human regulatory T cells and effector T cells cultured at standard 37C temperature and in mild hypothermia (33C).
Regulatory T cells (Tregs) play crucial role in maintenance of peripheral tolerance. Numerous clinical trials confirmed safety and efficacy of Treg treatment of for deleterious immune responses. However, Tregs lose their characteristic phenotype and suppressive potential during expansion ex vivo. In...
ORGANISM(S): Homo sapiens 
2017-10-04 | E-MTAB-5322 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of mouse embryonic stem cells, mesenchymal/neuronal progenitors and hepatocytes to identify epigenetic control of HOX gene termporal regulation
To reveal changes in chromatin state and correlate with transcriptional activity during embryonic development
ORGANISM(S): Mus musculus 
2009-06-05 | E-TABM-677 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human and mouse hematopoietic stem cells and other stages of the hematopoietic hierarchy, and to identify the shared expressed genes among the hematopoietic cells and neural and embryonic stem cells in mouse
Study to determine global gene expression profiles for mouse and human hematopoietic stem cells and other stages of the hematopoietic hierarchy, and to identify the shared expressed genes among the hematopoietic cells and neural and embryonic stem cells in mouse.
ORGANISM(S): Mus musculus 
2007-07-19 | E-CBIL-33 | biostudies-arrayexpress
TranscriptomicsmicroRNA signatures of iPSCs and endoderm-derived tissues
MicroRNAs (miRNAs), small non-coding RNAs that fine-tune gene expression, play multiple roles in the cell, including cell fate specification. We have analyzed the differential expression of miRNAs during fibroblast reprogramming into induced pluripotent stem cells (iPSCs) and endoderm induction in i...
ORGANISM(S): Mus musculus 
2015-02-20 | E-GEOD-36046 | biostudies-arrayexpress
TranscriptomicsTranscription Start Site (TSS) mapping and total RNAseq of Streptomyces scabies wild-type (WT) versus Streptomyces scabies ΔbtmL
The aim of this experiment was to map the transcription start sites (TSSs) in the bottromycin biosynthetic gene cluster from Streptomyces scabies, qualitatively assess the expression levels of this cluster within the bacterium's transcriptome and evaluate whether deletion of a potential regulatory g...
ORGANISM(S): Streptomyces scabiei 
2020-02-19 | E-MTAB-8236 | biostudies-arrayexpress
TranscriptomicsEffects of a short-term exposure to the fungicide prochloraz on endocrine function and gene expression in female fathead minnows (Pimephales promelas)
Prochloraz is a fungicide known to cause endocrine disruption through effects on the hypothalamic-pituitary-gonadal (HPG) axis. To determine the short-term impacts of prochloraz on gene expression and steroid production, adult female fathead minnows (Pimephales promelas) were exposed to the chemical...
ORGANISM(S): Pimephales promelas 
2011-02-03 | E-GEOD-26958 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Medicago roots from MtTi2i vs control after infection with Aphanomyces euteiches
Comparsion of root transcriptome profiles after Aphanomyces euteiches infections between control and MtTi2i-lines
ORGANISM(S): Medicago truncatula 
2006-03-03 | E-MEXP-572 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E. coli wild type and ycgF::kan cells treated with blue-light irradiation for 3 hours at 16oC
Wildtype and ycgF::kan cells were grown in LB medium at 37°C to an OD(578 nm) of 0,7. Then the cultures were shifted to 16°C and blue-light irradiated for 3 hours.
ORGANISM(S): Escherichia coli 
2009-06-03 | E-MEXP-1877 | biostudies-arrayexpress
TranscriptomicsEffect of interferon-gamma (IFNg) deficiency in murine small intestine (ileum)
Gene expression profiling of C57Bl/6 wild type and IFNg-deficient whole ileum at 8 weeks of age 5 wild type control, 5 IFNg knockout
ORGANISM(S): Mus musculus 
2016-07-16 | E-GEOD-69635 | biostudies-arrayexpress
GenomicsDNA double-strand breaks induced by high NaCl occur predominantly in gene deserts
High concentration of NaCl increases DNA breaks both in cell culture and in vivo. The breaks remain elevated as long as NaCl concentration remains high and are rapidly repaired when the concentration is lowered. Repair of the breaks after NaCl is reduced is accompanied by formation of foci containi...
ORGANISM(S): Mus musculus 
2011-11-21 | E-GEOD-32882 | biostudies-arrayexpress
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