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GenomicsHITS-CLIP analysis of FUS in lineage-committed cells.
In the present study, we performed HITS-CLIP analysis for FUS using mouse brain to extensively characterize tits RNA-binding sites and functional roles in RNA metabolisms. We identified preferential binding of FUS to stem-and-loop structures but without any discernible consensus motifs. FUS was pref...
ORGANISM(S): Mus musculus 
2012-07-26 | E-GEOD-37190 | biostudies-arrayexpress
TranscriptomicsExpression profiles of primary cortical neurons after knocking down Fus
To clarify the functional properties of FUS, we established the differentially expressed alternative exons in FUS-silenced primary cortical neurons by using exon-sensitive microarray technology. We analyzed total RNA of primary cortical neuron infected with lentivirus expressing shRNA against mouse ...
ORGANISM(S): Mus musculus 
2012-07-23 | E-GEOD-36153 | biostudies-arrayexpress
TranscriptomicsExon array analysis in the mouse myoblast cell line, C2C12 cells treated with control-, Cugbp1- or Mbnl1-siRNA
CUGBP1 and MBNL1 are developmentally regulated RNA-binding proteins that are causally associated with myotonic dystrophy type 1. Using HITS-CLIP anlysis, we found CUGBP1 and MBNL1 preferentially bind to alternatively spliced introns and exons, as well as to the 3' UTRs. To analyze more directly the...
ORGANISM(S): Mus musculus 
2012-01-03 | E-GEOD-29990 | biostudies-arrayexpress
TranscriptomicsmRNA decay analysis in the mouse myoblast cell line, C2C12 cells treated with conrtol-, Cugbp1- or Mbnl1-siRNA
CUGBP1 and MBNL1 are developmentally regulated RNA-binding proteins that are causally associated with myotonic dystrophy type 1. Using HITS-CLIP anlysis, we found CUGBP1 and MBNL1 preferentially bind to alternatively spliced introns and exons, as well as to the 3' UTRs. To analyze more directly the ...
ORGANISM(S): Mus musculus 
2012-01-03 | E-GEOD-27583 | biostudies-arrayexpress
TranscriptomicsRNA CLIP-seq for Cugbp1, Mbnl1 and Ptbp1 in mouse C2C12 myoblasts
CUG-binding protein 1 (CUGBP1) and muscleblind-like 1 (MBNL1) are developmentally regulated RNA-binding proteins that are causally associated with myotonic dystrophy type 1. We extensively determined RNA-binding sites of CUGBP1 and MBNL1 to investigate their roles in RNA processing. We also analyzed...
ORGANISM(S): Mus musculus 
2012-01-04 | E-MTAB-414 | biostudies-arrayexpress
TranscriptomicshnRNP A1 iCLIP
This experiment identifies hnRNP A1 binding sites transcriptome-wide in Hela cells. HeLa cells with inducible expression of T7-tagged hnRNP A1 were grown to approximately 90% confluence and then subject to iCLIP analysis (following the protocol from Huppertz et al. 2014 (iCLIP: protein-RNA interact...
ORGANISM(S): Homo sapiens 
2016-06-23 | E-MTAB-3612 | biostudies-arrayexpress
UnknownNeutrophils induce paracrine telomere dysfunction and senescence in ROS-dependent manner.
Not available
S-SCDT-EMBOJ-2020-106048P | biostudies-other
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