Sort   by:  
 Page size 
TranscriptomicsKlf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells
To characterize Klf4 downstream genes, we performed microarray analyses of the ES cells knocked down by siRNA of Klf4 gene. Keywords: individual genetic characteristic design ES cells grow without feeders on gelatin coated in complete ES medium. Cells were incubated at 37 0C; 5% CO2. ES cells were t...
ORGANISM(S): Mus musculus 
2010-06-10 | E-GEOD-5930 | biostudies-arrayexpress
TranscriptomicsIdentification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algor
We present a microarray experiment with the same tet-inducible system but with multiple time points within 24 hr to capture early responses to Oct4 suppression. These data were analyzed together with published genome-wide ChIP data. We found that most tentative target genes (TTG) of Oct4 in ES cells...
ORGANISM(S): Mus musculus 
2008-04-24 | E-GEOD-8617 | biostudies-arrayexpress
TranscriptomicsDissecting Oct3/4-regulated gene networks in embryonic stem cells by expression
POU transcription factor Pou5f1 (Oct3/4) is required to maintain ES cells in an undifferentiated state. Here we show that global expression profiling of Oct3/4-manipulated ES cells delineates the downstream target genes of Oct3/4. Combined with data from genome-wide chromatin-immunoprecipitation ass...
ORGANISM(S): Mus musculus 
2010-06-30 | E-GEOD-5936 | biostudies-arrayexpress
GenomicsTranscription factors interfering with dedifferentiation induce direct conversion
Although transcription factor(TF)s regulate differentiation-related processes, including dedifferentiation and direct conversion, functional interactions between TFs regulating these processes are not well understood. Here we show that TFs preventing dedifferentiation are able to induce direct conve...
ORGANISM(S): Mus musculus 
2013-01-01 | E-GEOD-41252 | biostudies-arrayexpress
TranscriptomicsPluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells
To characterize the differentiation by Sox2 KO, we performed microarray analyses of mouse ES cell line 2TS22C during the time-course being induced of Sox2 KO Inducible Sox2 KO cell line 2TS22C were assigned to eight experimental groups and cultured in GMEM medium (SIGMA) with LIF at 37°C in an atmo...
ORGANISM(S): Mus musculus 
2010-06-11 | E-GEOD-5895 | biostudies-arrayexpress
TranscriptomicsRNAi knock down of Klf4 in mouse embryonic stem cells
To characterize Klf4 downstream genes, we performed microarray analyses of the ES cells knocked down by siRNA of Klf4 gene.
ORGANISM(S): Mus musculus 
2006-11-08 | E-MNIA-92 | biostudies-arrayexpress
Sort   by:  
 Page size