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TranscriptomicsGene expression profiling of IOERT exposed MCF10A breast epithelial cells
Intraoperative Electron Radiation Therapy (IOERT) is a therapeutic technique that delivers a single high dose of ionizing radiation (IR) directly to the tumor bed during cancer surgery. The main goal of IOERT is to counteract tumor growth by acting on residual cancer cells in the site at high risk f...
ORGANISM(S): Homo sapiens 
2015-07-06 | E-GEOD-65954 | biostudies-arrayexpress
TranscriptomicsGene Expression in MCF10A cells through Differentiation on Transwells
To further understand the differences occurring in MCF10A cells as they polarize and differentiate in the Transwell® model, we performed gene expression profiling with Affymetrix Human Genome U133 Plus 2.0 Arrays. Four experimental time points, were sampled: conventional cultures of MCF10A cells ...
ORGANISM(S): Homo sapiens 
2008-06-11 | E-GEOD-10070 | biostudies-arrayexpress
TranscriptomicsC/EBPbeta-2 regulation of gene expression in MCF10A cells
C/EBPbeta-2 results in EMT and ErbB indpendence this project investigated the gene changes in related genes upon C/EBPbeta-2 overexpression in MCF10A cells. We used microarray analysis to detail the global gene expression mediated by C/EBPbeta-2 and identified changes in known EMT genes, however, k...
ORGANISM(S): Homo sapiens 
2010-03-06 | E-GEOD-15065 | biostudies-arrayexpress
TranscriptomicsGene expression changes as a result of E-cadherin loss in an isogenic non-malignant MCF10A and MCF10A CDH1-/- breast cells
Background: E-cadherin is an adherens junction protein that forms homophilic intercellular contacts in epithelial cells while also interacting with the intracellular cytoskeletal networks. It has roles including establishment and maintenance of cell polarity, differentiation, migration and signallin...
ORGANISM(S): Homo sapiens 
2014-08-01 | E-GEOD-59317 | biostudies-arrayexpress
TranscriptomicsGene expression profiling of MCF10A (miR-221/222 vs control)
Analysis of gene expression of MCF10A to identify the targets of miR-221 and miR-222 Keywords: MCF10, miR-221, miR-222 RNA profiles of human MCF10A cell line
ORGANISM(S): Homo sapiens 
2011-06-14 | E-GEOD-29327 | biostudies-arrayexpress
TranscriptomicsSUMO2 ChIP-seq in MCF10A cells
Chromatin immuno-precipitation using SUMO2 (Life Technologies) antibody in MCF10A cell line, treated with epidermal growth factor for 30 minutes.
ORGANISM(S): Homo sapiens 
2019-07-16 | E-MTAB-7759 | biostudies-arrayexpress
TranscriptomicsmRNA-seq of MCF10A USP22 knockdown cells
This analysis identifies genes which are affected in MCF10A cells upon siRNA-mediated USP22 knockdown
ORGANISM(S): Homo sapiens 
2019-12-06 | E-MTAB-8247 | biostudies-arrayexpress
TranscriptomicsTimecourse total transcriptome sequencing of MCF10A cells undergoing acinus formation.
RNA-seq was performed for transcriptional analysis of MCF10A cells, an epithelial mammary cell line. MCF10A cells were cultured in 3D acinus forming conditions (in Matrigel). Timepoints analysed were 24h, 34h, 36h, 38h and 48h into acinus formation. Control cells were monoloayer.
ORGANISM(S): Homo sapiens 
2014-10-02 | E-MTAB-2969 | biostudies-arrayexpress
TranscriptomicsGene expression profiling in Human mammary epithelial cell (MCF10A) by repression of KRT81 expression
To investigate new health functions of hair keratin KRT81 in MCF10A, we compare altered of the genes expression. The expression of KRT81 was blocked by transient transfection with KRT81 siRNA in MCF10A cells.
ORGANISM(S): Homo sapiens 
2016-08-06 | E-GEOD-85236 | biostudies-arrayexpress
TranscriptomicsGene Expression Profiling on Stably Expressing ICD JM-a CYT-1 or CYT-2 ERBB4 isoforms in MCF10A cells
Convergent and divergent effects of two ICD ERBB4 isoforms on gene expression in normal-like MCF10A mammary epithelial cells lacking endogeneous ERBB4 expression. Control vector (VEC) and ERBB4 expression MCF10A cells (ICD CYT1 or CYT2) maintained in regular medium containing 10% serum, were collect...
ORGANISM(S): Homo sapiens 
2014-05-07 | E-GEOD-57339 | biostudies-arrayexpress
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