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RNA structural transitions are important in the function and regulation of RNAs. Here, we reveal a layer of transcriptome organization in the form of RNA folding energies. By probing yeast RNA structures at different temperatures, we obtained relative melting temperatures (Tm) for RNA structures in ...
ORGANISM(S): Saccharomyces cerevisiae 
The use of DNA microarrays to identify nucleotide variation is almost 20 years old. A variety of improvements in probe design and experimental conditions have brought this technology to the point that single-nucleotide differences can be efficiently detected in unmixed samples, although developing r...
ORGANISM(S): Saccharomyces cerevisiae 
We have developed a mass spectrometry-based approach that allowed us to quantitatively monitor protein stability across a broad range of temperatures at the proteome scale (meltome). We profiled the meltomes of several microorganisms and eukaryotic species including human, allowing to investigate th...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) Picrophilus torridus Oleispira antarctica Arabidopsis thaliana (Mouse-ear cress) Mus musculus (Mouse) Drosophila melanogaster (Fruit fly) Caenorhabditis elegans Geobacillus stearothermophilus Homo sapiens (Human) Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) Bacillus subtilis 
2020-04-29 | PXD011929 | Pride
Plants regulate their time to flowering by gathering information from the environment. Photoperiod and temperature are among the most important environmental variables. Suboptimal, but not near-freezing, temperatures regulate flowering through the thermosensory pathway, which overlaps with the auton...
ORGANISM(S): Arabidopsis thaliana 
The thermal shift assay is a robust method of discovering protein-ligand interactions by measuring the alterations in protein thermal stability under various conditions. Several thermal shift assays have been developed and their throughput has been advanced greatly by the rapid progress in tandem ma...
ORGANISM(S): Homo sapiens (Human) 
2020-04-06 | PXD017346 | Pride
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