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TranscriptomicsGene expression profile of Dicer knockout in mouse DP thymocytes
The RNase III enzyme dicer is essential for the processing of microRNAs (miRNAs) and small interfering RNAs (siRNAs) from double-stranded RNA precursors. miRNAs and siRNAs regulate chromatin structure, gene transcription, mRNA stability and translation in a wide range of organisms. To provide a mode...
ORGANISM(S): Mus musculus 
2015-01-28 | E-GEOD-57511 | biostudies-arrayexpress
GenomicsMicroRNAs maintain bivalency in mouse embryonic stem cells [ChIP-seq]
The protein Dicer is required for microRNA (miRNA) biogenesis, and therefore Dicer-deficient cells lack all mature, functional miRNAs. Here we investigated the binding of the PRC2 component Ezh2 in wildtype and Dicer-deficient mouse embryonic stem cells genomewide using ChIP-sequencing analysis. Exa...
ORGANISM(S): Mus musculus 
2016-06-02 | E-GEOD-60160 | biostudies-arrayexpress
TranscriptomicsA Myc-dependent role for cohesin in the heterokaryon-mediated reprogramming of somatic nuclei
Analysis of gene expression changes in mESCs where the cohesin subunit Scc1 has been depleted. There are 6 samples. 3 Scc1 wt/wt ERT2-Cre biological replicas and 3 Scc1 lox/lox ERT2-Cre biological replicas, all treated with 4'-OHT 80nM final concentration for 24h. Scc1 wt/wt and Scc1 lox/lox ERT2-Cr...
ORGANISM(S): Mus musculus 
2015-02-19 | E-GEOD-62325 | biostudies-arrayexpress
TranscriptomicsGene expression in mouse primary pre-B cells transduced with Ikaros
Ikaros family DNA binding proteins are critical regulators of B cell development. To identify Ikaros-regulated genes in primary pre-B cells we performed gene expression microarrays. We used retroviral gene transfer to express Ikaros proteins. Total RNA from 3 biological replicates of primary pre-B c...
ORGANISM(S): Mus musculus 
2013-01-15 | E-GEOD-42551 | biostudies-arrayexpress
TranscriptomicsGene expression in mouse pre-B cells transduced with Ikaros.
Ikaros family DNA binding proteins are critical regulators of B cell development. To identify Ikaros-regulated genes in pre-B cells we performed gene expression studies at enhanced temporal resolution. We used retroviral gene transfer to express Ikaros proteins in the pre-B cell line B3. Total RNA f...
ORGANISM(S): Mus musculus 
2013-01-15 | E-GEOD-38110 | biostudies-arrayexpress
GenomicsGenome-wide identification of Ikaros binding sites in primary pre-B cells
Ikaros DNA binding proteins are important regulators of haematopoiesis and genetic deletion of Ikaros results in severe developmental disturbances, including delayed thymocyte differentiation and an early and complete block in B cell development. Although Ikaros ChIP-seq data are available for mouse...
ORGANISM(S): Mus musculus 
2013-01-15 | E-GEOD-42462 | biostudies-arrayexpress
GenomicsGenome-wide identification of Ikaros binding sites in pre-B cells
Ikaros DNA binding proteins are important regulators of haematopoiesis and genetic deletion of Ikaros results in severe developmental disturbances, including delayed thymocyte differentiation and an early and complete block in B cell development. Although Ikaros ChIP-seq data are available for mouse...
ORGANISM(S): Mus musculus 
2013-01-15 | E-GEOD-38169 | biostudies-arrayexpress
TranscriptomicsExpression data from wildtype and Dicer-deficient mouse embryonic stem (ES) cells at different stages of the cell cycle.
The protein Dicer is required for microRNA (miRNA) biogenesis. Dicer-deficient cells therefore lack almost all mature, functional miRNAs. We investigated the role of miRNAs in regulation of gene expression in mouse ES cells by analysing gene expression in either wildtype or Dicer-deficient cells gro...
ORGANISM(S): Mus musculus 
2016-06-02 | E-GEOD-60096 | biostudies-arrayexpress
TranscriptomicsComparison of miRNA profile of natural T reg cells with conventional CD4+CD25- T cells
Low molecular weight RNA from conventional T cells and T reg cells was hybridised to microarrays containing oligonucleotide probes corresponding to known miRNA sequences. Keywords: cell type comparison Six arrays representing three biological replicates and dyeswaps of each.
ORGANISM(S): Mus musculus 
2010-07-01 | E-GEOD-6003 | biostudies-arrayexpress
TranscriptomicsComparison of miRNA profile of Foxp3-transduced activated T cells with control vector-transduced activated T cells
Activated T cells were transduced with either Foxp3-IRES-GFP or IRES-GFP control vector. Low molecular weight RNA from cells was extracted 72h or 96h later and hybridised to microarrays containing oligonucleotide probes corresponding to known miRNA sequences. Keywords: cell type comparison Four arra...
ORGANISM(S): Mus musculus 
2010-07-01 | E-GEOD-6007 | biostudies-arrayexpress
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