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MetabolomicsMetabolomic Responses of Guard Cells and Mesophyll Cells to Bicarbonate
Anthropogenic CO2 presently at 400 ppm is expected to reach 550 ppm in 2050, an increment expected to affect plant growth and productivity. Paired stomatal guard cells (GCs) are the gate-way for water, CO2, and pathogen, while mesophyll cells (MCs) represent the bulk cell-type of green leaves mainly...
2017-03-31 | MTBLS309 | MetaboLights
MetabolomicsThe timing of bacterial mesophyll infection shapes the leaf chemical landscape during the interaction

Chemistry in eukaryotic intercellular spaces is shaped by both hosts and symbiotic microorganisms such as bacteria. Pathogenic microorganisms like barley associated Xanthomonas translucens (Xt) swiftly overtake the inner leaf tissue becoming the dominant microbial community member during disease ...

2024-02-01 | MTBLS7676 | MetaboLights
MetabolomicsApoplastic metabolomics reveals sugars as mesophyll messengers regulating guard cell ion transport under red light
Here we sought to identify mesophyll messenger(s) that promote stomatal opening under red light. We first established a method to isolate leaf apoplastic fluids from Arabidopsis thaliana and Vicia faba and ascertained that the apoplastic fluid enhances red light-induced stomatal opening. We then app...
2025-06-23 | MTBLS5383 | MetaboLights
ProteomicsDifferences in proteasome targeting in Arabidopsis leaf mesophyll, epidermal and vascular cells
For the detailed and sensitive detection of the responses to a specific treatment it is important to perform tissue or cell type specific analyses, but this is not easily achievable for the different leaf tissues. Here we developed a method termed MeSelect to effectively separate leaf epidermis, vas...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2015-05-13 | PXD000942 | Pride
Ubiquitylation Arabidopsis Syringolin a Affinity enrichment Vasculature Mesophyll Ubiquitin proteasome system Leaf tissues Quantitative proteomics Epidermis 
TranscriptomicsTranscription profiling by array of Arabidopsis guard cells and mesophyll cells in response to ABA treatment
Transcriptional profiling of guard cells and mesophyll cells in response to ABA treatment
ORGANISM(S): Arabidopsis thaliana 
2008-02-02 | E-MEXP-1443 | biostudies-arrayexpress
ProteomicsPeeling back the layers: Functional differentiation between Kalanchoë fedtschenkoi epidermis and mesophyll proteomes
What proteins exist in the epidermis layer and how they compare to the proteins in the surrounding mesophyll cells represents a major knowledge gap for how CAM plants thrive in environments with high temperatures and long periods of severe water deficit. Therefore, we performed large-scale proteomic...
ORGANISM(S): Kalanchoe fedtschenkoi 
2020-04-02 | PXD010837 | Pride
Cam Epidermal peel Shotgun proteomics Guard cell Mesophyll Plant proteomics 
ProteomicsDifferences in proteasome targeting in Arabidopsis leaf mesophyll, epidermal and vascular cells
For the detailed and sensitive detection of the responses to a specific treatment it is important to perform tissue or cell type specific analyses, but this is not easily achievable for the different leaf tissues. Here we developed a method termed MeSelect to effectively separate leaf epidermis, vas...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2015-05-14 | PXD000941 | Pride
Ubiquitylation Arabidopsis Syringolin a Affinity enrichment Vasculature Mesophyll Ubiquitin proteasome system Leaf tissues Quantitative proteomics Epidermis 
TranscriptomicsGene expression changes underlying initial cellular and molecular responses to MAMPs in Arabidopsis mesophyll cells
This SuperSeries is composed of the following subset Series: GSE16471: Identification of CPK5ac and CPK11ac early target genes in Arabidopsis mesophyll cells GSE16472: Identification of early flg22 responsive genes in Arabidopsis mesophyll cells GSE17382: Identification of early flg22 responsive gen...
ORGANISM(S): Arabidopsis thaliana 
2010-03-18 | E-GEOD-16557 | biostudies-arrayexpress
TranscriptomicsIdentification of early flg22 responsive genes in Arabidopsis mesophyll cells
The goal of this experiment was to identify the early responsive genes activated by the 22 amino acid peptide of bacterial flagellin (flg22) in Arabidopsis mesophyll cells that are involved in the initial responses important for plant innate immunity. We used the microarrays to detail the global pro...
ORGANISM(S): Arabidopsis thaliana 
2010-03-18 | E-GEOD-16472 | biostudies-arrayexpress
TranscriptomicsIdentification of CPK5ac and CPK11ac early target genes in Arabidopsis mesophyll cells
The goal of this experiment was to identify the early target genes of constitutively activated CPK5ac and CPK11ac in Arabidopsis mesophyll cells that are involved in early flagellin responses important for plant innate immunity. We used the microarrays to detail the global program of gene expression...
ORGANISM(S): Arabidopsis thaliana 
2010-03-18 | E-GEOD-16471 | biostudies-arrayexpress
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