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TranscriptomicsTranscription profiling of immature germinal vesicle stage oocytes with mature metaphase II oocytes to identify and characterize the changed and stable transcripts during mouse oocyte maturation.
There is massive destruction of transcripts during maturation of mouse oocytes. The objective of this project was to identify and characterize the transcripts that are degraded versus those that are stable during the transcriptionally silent germinal vesicle (GV)-stage to metaphase II (MII)-stage t...
ORGANISM(S): Mus musculus 
2007-11-13 | E-GEOD-5668 | biostudies-arrayexpress
TranscriptomicsStorage time does not modify the gene expression profile of cryopreserved human metaphase II oocytes
Study question: Does storage time impact on transcriptome of slowly frozen cryopreserved human metaphase II (MII) oocytes? Summary answer: For the first time, we demonstrate that the length of cryostorage has no effect on the gene expression profile of human metaphase II oocytes. What is known alrea...
ORGANISM(S): Homo sapiens 
2015-09-19 | E-GEOD-69768 | biostudies-arrayexpress
ProteomicsHuman oocyte proteome and secretome
The proteome and secretome of human oocytes was studied by shotgun LCMSMS, starting from pools of 100 cells. Exploiting SP3, a novel technology for proteomic sample preparation using magnetic beads, we scaled down proteome analysis to single cells. Despite the low protein content of only ~100ng per...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-11-15 | MSV000088387 | MassIVE
TranscriptomicsExpression data from human cumulus cells isolated from oocytes at MI and MII stages in polycystic ovary syndrome (PCOS) patients
Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder that is characterized by increased circulating androgen levels, anovulatory infertility, and frequently, insulin resistance and hyperinsulinemia.The abnormity of oocyte nuclear maturity is the main reason for anovulatory i...
ORGANISM(S): Homo sapiens 
2013-06-05 | E-GEOD-40400 | biostudies-arrayexpress
TranscriptomicsContrast of poly-ribosomal mRNA in germina vesicle (GV) and metaphase II (MII) bovine oocytes.
Poly-ribosomal mRNA from pools of 75 bovine oocytes at the germinal vesicle(GV) and metaphase II(MII) stages were isolated by sucrose fractionation then compared through microarray hybridization. 3 biological replicates from germinal vesicle(GV) and metaphase II(MII) bovine oocytes were hybridized a...
ORGANISM(S): Bos taurus 
2014-09-09 | E-GEOD-56603 | biostudies-arrayexpress
TranscriptomicsRNA-Seq profiling of single bovine oocyte transcript abundance and its modulation by cytoplasmic polyadenylation
Polyadenylated RNA from individual germinal vesicle and metaphase II ooyctes was amplified and processed for Illumina sequencing. Increases in polyadenylated transcript abundance were observed and are likely due to cytoplasmic polyadenylation. Transcriptomic analysis of GV and MII bovine oocytes.
ORGANISM(S): Bos taurus 
2015-07-08 | E-GEOD-61717 | biostudies-arrayexpress
TranscriptomicsComparison of the human oocyte to its sister polar body
Clinicians need additional metrics for predicting quality of human oocytes for IVF procedures. Human polar bodies reflect the oocyte transcript profile. Quantitation of polar body mRNAs could allow for both oocyte ranking and embryo preferences in IVF applications. The transcriptome of a polar body ...
ORGANISM(S): Homo sapiens 
2011-10-12 | E-GEOD-32689 | biostudies-arrayexpress
TranscriptomicsTranscription profiles of cumulus cells from GnRH agonists and GnRH antagonists treated oocytes at different maturity stages
Affymetrix gene expression profiling in cumulus cells (CC) retrieved from patients undergoing GnRH agonists and GnRH antagonists IVF treatment. Oocytes from three different maturity stages were considered: metaphase I oocytes (MI), nonfertilized metaphase II (MII) oocytes (MII-NF) and MII oocytes de...
ORGANISM(S): Homo sapiens 
2013-09-13 | E-GEOD-34230 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse in vivo matured MII oocytes and fully in vivo grown germinal vesicle oocytes to identify gene transcripts linked to epigenetic reprogramming
To elucidate some of the tools involved in early embryonic reprogramming, the levels of gene transcripts believed to be of importance to epigenetic modifications, and chromatin remodeling were detected by oligonucleotide microarrays in in vivo matured MII oocytes and compared with fully in vivo grow...
ORGANISM(S): Mus musculus 
2007-07-02 | E-MEXP-1146 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human mature oocytes in relation to age
Background: The development competence of human oocytes declines with increasing age. The objective of this study was to investigate the effect of age on gene expression profile in mature human oocytes.
Methods: mRNA was isolated for whole genome gene expression microarray analysis from metaphase...
ORGANISM(S): Homo sapiens 
2009-12-31 | E-MEXP-2347 | biostudies-arrayexpress
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