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TranscriptomicsATAC-seq in the U2OS-AR cell line comparing cells treated for 4 hours with 5 nM R1881 to vehicle (DMSO) control-treated cells
We performed ATAC-seq in the U2OS-AR cell line (derived from U2OS ATTC:HTB-96, stably transfected with an expression construct for human AR), upon androgen (R1881) or vehicle (DMSO) treatment for 4 hours.
ORGANISM(S): Homo sapiens 
2021-03-09 | E-MTAB-9606 | biostudies-arrayexpress
TranscriptomicsGene expression of LNCaP cells grown in polyethlene glycol-based hydrogels alone (monocultures) and grown with human osteoblasts as tissue engineered bone (co-culture) with the presence of synthetic androgen, R1881
Analysis of LNCaP cell molecular differences in monocultures and in co-cultures in the presence of R1881. Human osteoblast molecular signatures were also identified from the tissue engineered bone monocultures. LNCaP cells molecular profile was altered by co-culturing with human osteoblasts compared...
ORGANISM(S): Homo sapiens 
2014-05-13 | E-GEOD-44143 | biostudies-arrayexpress
GenomicsA Role for WDR5 in Integrating Threonine 11 Phosphorylation to Lysine 4 Methylationon Histone H3 and in Prostate Cancer
Upon androgen stimulation, PKN1-mediated histone H3 threonine 11 phosphorylation (H3T11P) promotes AR target genes activation. However, the underlying mechanism is not completely understood. Here, we show that WDR5, a subunit of the SET1/MLL complex, interacts with H3T11P and this interaction facili...
ORGANISM(S): Homo sapiens 
2014-07-01 | E-GEOD-55279 | biostudies-arrayexpress
TranscriptomicsGene expression of LNCaP cells grown in 2D cultures and polyethlene glycol-based hydrogels with the presence of synthetic androgen, R1881
Analysis of LNCaP cell molecular differences and their response to R1881 in 2D and 3D cultures. Androgen regulated genes were differentially expressed between 2D and 3D cultures. These results provide insights into factors that influence the expression of androgen regulated genes In this study, LNCa...
ORGANISM(S): Homo sapiens 
2013-02-04 | E-GEOD-33610 | biostudies-arrayexpress
TranscriptomicsRNA-seq in the U2OS-AR cell line to identify genes regulated by the androgen receptor
To identify the genes regulated by the androgen receptor (AR), we performed RNA-seq in the U2OS-AR cell line (derived from U2OS ATTC:HTB-96, stably transfected with an expression construct for human AR), upon androgen (R1881) or vehicle (DMSO) treatment for 24 hours.
ORGANISM(S): Homo sapiens 
2021-03-09 | E-MTAB-9622 | biostudies-arrayexpress
TranscriptomicsA genome-wide and dose-dependent inhibition map of androgen receptor binding by small molecules reveals its regulatory program upon antagonism
The androgen receptor plays a critical role throughout the progression of prostate cancer and is an important drug target for this disease. While chromatin immunoprecipitation coupled with massively parallel sequencing (ChIP-Seq) is becoming an essential tool in studying transcription and chromatin...
ORGANISM(S): Homo sapiens 
2013-12-31 | E-GEOD-32892 | biostudies-arrayexpress
ProteomicsComparative proteomic and transcriptomic analysis of the impact of androgen stimulation and darolutamide inhibition on prostate cancer cells
Androgen receptor (AR) signaling is a major driver and therapy target in prostate cancer. Several inhibitors of AR function are approved for different stages of the disease and their impact on downstream gene transcription has been described. However, the ensuing effects of androgen and anti-androge...
ORGANISM(S): Homo sapiens (Human) 
2023-02-17 | PXD036962 | Pride
Andrigen receptor signalling Vcap Darolutamide 
TranscriptomicsIdentification of direct androgen-target genes in the prostate cancer cell line LNCaP
Transcriptional profiling of the LNCaP cell line treated with R1881 synthetic androgen and/or cycloheximide. LNCaP cells were stimulated with R1881 alone, with cycloheximide alone, or with R1881 in the presence of cycloheximide for 24h and compared to control untreated cells. Two independent cell cu...
ORGANISM(S): Homo sapiens 
2014-06-14 | E-GEOD-22372 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of human LNCaP cells treated with synthetic androgen R1881 or ethanol (vehicle) using androgen receptor antibody
LNCaP cells were grown in charcoal stripped media for 48hrs and then treated for 1hr with the synthetic androgen R1881 or ethanol (vehicle). Cell extracts from both treatment conditions were then subjected to chromatin-immunoprecipitation using an anti-androgen receptor antibody, before labeling and...
ORGANISM(S): Homo sapiens 
2007-08-17 | E-TABM-233 | biostudies-arrayexpress
TranscriptomicsLong-term androgen withdrawal and treatment (R1881) of LNCaP-FGC prostate cancer cells
LNCaP cells were cultures in steroid depleted medium for 5 days before treatment with synthetic androgen (R1881, 10nM) for 16h. Transcriptomics analysis was performed to compare gene expression changes induced by androgen withdrawal or androgen treatment. Genome-wide transcriptomic analysis of LNCaP...
ORGANISM(S): Homo sapiens 
2013-10-22 | E-GEOD-51460 | biostudies-arrayexpress
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