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Mechanisms by which G-patch activators tune the processive multi-tasking ATP-dependent RNA helicase Prp43 (DHX15 in humans) to productively remodel diverse RNA:protein complexes remain elusive. Here, a comparative study between a new activator, Tma23, and a characterized activator, Pxr1, uncovered s...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-10-09 | PXD048382 | Pride
Mechanisms by which G-patch activators tune the processive multi-tasking ATP-dependent RNA helicase Prp43 to remodel diverse RNA:protein complexes remain elusive. Here, a comparative study between a new activator, Tma23, and a characterized activator, Pxr1, uncovered segments that organize Prp43 fun...
ORGANISM(S): Saccharomyces cerevisiae Saccharomyces cerevisiae (Baker's yeast) 
2024-10-09 | PXD046892 | Pride
Short nascent strands purification coupled to next-generation sequencing allowed us to identify replication origins on human genome in an extensive way, by mapping replication origins in 4 different cell types, IMR-90 fibroblasts, hESC H9 cells, iPSC Th Cl-4 cells and HeLa cells. We demonstrated the...
ORGANISM(S): Homo sapiens 
Transposon insertion site sequencing (TIS) is a powerful tool that has significantly advanced our knowledge of functional genomics. While providing valuable insights, these applications of TIS focus on (conditional) gene essentiality and neglect possibly interesting but subtle differences in the imp...
ORGANISM(S): Saccharomyces cerevisiae 
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