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TranscriptomicsExpression data from mouse insulinoma cells
We previously isolated a subclone, MIN6 clone 4, from the parental MIN6 cells, that shows well-regulated insulin secretion in response to glucose, glybenclamide, and KCl, even after prolonged culture. To investigate the molecular mechanisms responsible for preserving GSIS in this subclone, we compar...
ORGANISM(S): Mus musculus 
2013-04-22 | E-GEOD-43774 | biostudies-arrayexpress
TranscriptomicsGene expression data from Min6 cells grown in serum containing and serum free condtions following Hes3 knock down
The basic helix-loop-helix (bHLH) transcription factor hairy and enhancer of split (Hes3) is a member of the Hes/Hey gene family that regulates developmental processes in progenitor cells from various tissues. We demonstrated the Hes3 expression in mouse pancreatic tissue, suggesting it may have a r...
ORGANISM(S): Mus musculus 
2014-12-23 | E-GEOD-64449 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse pancreatic beta cell line (MIN6) treated with amylin at different times and doses and growth at different concentrations of glucose
Murine pancreatic beta cell line MIN6 was growth at two different concentrations of glucose (22,2 and 5,5 mM of glucose), 37ºC, 5% CO2 and was treated at four different concentrations of human amylin (0, 1, 10 and 20 uM) during three different times (2, 12 and 24 hours)
ORGANISM(S): Mus musculus 
2009-04-10 | E-GEOD-2253 | biostudies-arrayexpress
TranscriptomicsExpression data from Min6 cells maintained under different culture conditons that affect Hes3 expression and localization
The basic helix-loop-helix (bHLH) transcription factor hairy and enhancer of split (Hes3) is a member of the Hes/Hey gene family that regulates developmental processes in progenitor cells from various tissues. We demonstrated the Hes3 expression in mouse pancreatic tissue, suggesting it may have a r...
ORGANISM(S): Mus musculus 
2014-12-23 | E-GEOD-64444 | biostudies-arrayexpress
TranscriptomicsRNA-seq of MIN6 cells overexpressing Hnf1a through CRISPR activation
To determine the impact of Hnf1a overexpression on gene expression in mouse beta cells, we performed RNA-seq experiments on MIN6 where Hnf1a was overexpressed using CRISPR activation (SAM system).
ORGANISM(S): Mus musculus 
2022-05-01 | E-MTAB-11474 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse MIN6 insulinoma beta cells following knock-down of murine Pdx1
In order to identify genes that are regulated by Pdx1, MIN6 insulinoma cells were transduced with adenovirus expressing a short hairpin RNA directed against murine Pdx1 (AdshPdx1) or luciferase (AdshLuc) or not transduced at all (untreated). RNA was harvested and reverse transcribed. Reduced expre...
ORGANISM(S): Mus musculus 
2010-01-01 | E-MTAB-132 | biostudies-arrayexpress
TranscriptomicsBeta cell 5’-shifted isomiRs are candidate regulatory hubs in type 2 diabetes
We performed deep sequencing of small RNA from mouse insulinoma (MIN6) cells cultured in 25mM glucose. We then developed and implemented an in-house short-read mapping strategy to analyze isomiR diversity. Profile of miRNA expression in MIN6 cells cultured in 25mM glucose.
ORGANISM(S): Mus musculus 
2013-09-10 | E-GEOD-44262 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of mouse beta cell line MIN6 after siRNA knockdown of lncRNA Pax6os1
Pax6os1 was knocked using by a pool of 3 siRNAs in the mouse insulinoma (beta cell) line MIN6. A parallel experiment using 3 control siRNAs was performed and RNA extracted 72 hours after transfection and subjected to RNA-Seq.
ORGANISM(S): Mus musculus 
2021-06-01 | E-MTAB-9218 | biostudies-arrayexpress
TranscriptomicsMicroarray Analysis of MIN6 cells treated with Exendin-4 over time
The aim of the study was to investigate the effect of Exendin-4 on MIN6 cells allowing for the elucidation of the various transcriptional programs initiated by this multifunctional peptide hormone. MIN6 cells were treated with Exendin-4 for 24, 48 and 72 hours. After these time points RNA was isolat...
ORGANISM(S): Mus musculus 
2014-11-04 | E-MTAB-3071 | biostudies-arrayexpress
TranscriptomicsMeg3 regulated genes in pancreatic beta cells
Meg3 is a long non-coding RNA. It's target genes are unknown. The mouse pancreatic beta cell line MIN6-4N was used to assess the expression of genes upon stable Meg3 overexpression Stable cell lines were isolated that have integated pcDNA3.1 or pcDNA3.1-mMeg3.The cells were processed for RNA isolati...
ORGANISM(S): Mus musculus 
2015-05-21 | E-GEOD-57716 | biostudies-arrayexpress
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