Here, we introduce a reversible protein tag, ProMTag, that enables whole proteome capture, cleanup, and release of intact proteins for top-down analysis. Alternatively, the addition of a novel Trypsin derivative to the workflow generates peptides for bottom-up analysis. We show that the ProMTag work...
ORGANISM(S): Burkholderia sp. 2332 Saccharomyces cerevisiae (Baker's yeast) Gallus gallus (Chicken)
