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Purified protein complexes were separated by SDS-PAGE and gel lanes divided into 3 sections (A, B and C). Samples prepared in triplicate (1, 2 and 3). A nanoElute (Bruker) was attached in line to a timsTOF Pro equipped with a CaptiveSpray Source (Bruker, Hamburg, Germany). Chromatography was conduct...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
Rhodobacter sphaeroides produces hydrogen gas (H2) via its nitrogenase enzyme during photoheterotrophic growth under nitrogen-limited conditions. We find that cells produce different amounts of H2 and show different growth rates, depending on the organic substrate provided (lactate, succinate, gluc...
ORGANISM(S): Rhodobacter sphaeroides 
As a result of ancestral whole genome and small-scale duplication events, the genome of Saccharomyces cerevisiaeM-bM-^@M-^Ys, and of many eukaryotes, still contain a substantial fraction of duplicated genes. In all investigated organisms, metabolic pathways, and more particularly glycolysis, are spe...
ORGANISM(S): Saccharomyces cerevisiae 
Effects of the prop-1 and Ghrhr mutations in gene expression during normal aging in mice.
ORGANISM(S): Mus musculus 
Strains devoid of ppGpp (ΔrelA ΔspoT; called ppGpp0), and ppGpp0 dksA- exhibit several amino acid requirements for growth on minimal media. We found that overexpression of DksA can complement some of those requirements. Since DksA is a factor that binds to the RNA polymerase secondary channel, we wo...
ORGANISM(S): Escherichia coli 
Complex biological processes are often regulated, at least in part, by the binding of transcription factors to their targets. Recently, considerable effort has been made to analyze the binding of relevant factors to the suite of targets they regulate, thereby generating a regulatory circuit map. H...
ORGANISM(S): Saccharomyces cerevisiae 
The RpoS sigma factor protein of Escherichia coli is the master transcriptional regulator of physiological responses to a variety of stresses. This stress response comes at the expense of scavenging for scarce resources, causing a trade-off between stress tolerance and nutrient acquisition. This t...
ORGANISM(S): Escherichia coli 
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