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TranscriptomicsHuman mitochondria hub of small RNAs: Analysis by deep Sequencing
We report here that human mitochondria contain small RNA including microRNA, piRNA, tRNA, rRNA, and RNA repeats. Mitochondria from human cells were purified and RNA isolated. Small RNAs were purified, library generated and analyzed by Illumina Hiseq 2000 system. The sequencing generated 19.5 and 17....
ORGANISM(S): Homo sapiens 
2013-03-05 | E-GEOD-32185 | biostudies-arrayexpress
ProteomicsRapid single-step affinity purification of HA-tagged mitochondria from Arabidopsis thaliana
Photosynthesis in plant cells would not be possible without the supportive role of mitochondria. However, isolating mitochondria for physiological and biochemical analyses from plant cells is a lengthy and tedious process. Established isolation protocols require multiple centrifugation steps and sub...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-11-14 | PXD014137 | Pride
Arabidopsis Mitochondria Lc-ms/ms 
ProteomicsMceC affinity purification and interactome analysis
Anti-FLAG affinty purification of mitochondria isolated from tetracycline inducible HEK293 cells expressing MceC-3XFLAG or control (EV) cells. Equal amounts of mitochondrial protein were solubilised and anti-FLAG immunoprecipitation performed.
ORGANISM(S): Homo sapiens (Human) Coxiella burnetii 
2020-11-12 | PXD019417 | Pride
Coxiella burnetii Effector protein Mitochondria 
ProteomicsProteomics of Dictyostelium discoideum mitochondria
There is a paucity of data regarding what proteins localize to the mitochondria in the social amoeba Dictyostelium discoideum. Therefore, here, we utilized high-throughput tandem mass tag (TMT)-based protein quantitation to identify proteins in the whole-cell lysate, as well as in crude and purified...
ORGANISM(S): Dictyostelium discoideum (Slime mold) 
2022-06-06 | PXD029101 | Pride
Dictyostelium discoideum Proteomics Tmt Mitochondria purification Lcmsms 
TranscriptomicsHeLa cell mitochondrial microRNAs
MicroRNAs (miRNAs) are small non-coding RNAs that associate with Argonaute 2 protein to regulate gene expression at the post-transcriptional level in the cytoplasm. However, recent studies have reported that some miRNAs localize to and function in other cellular compartments. Mitochondria harbour th...
ORGANISM(S): Homo sapiens 
2011-10-05 | E-GEOD-24761 | biostudies-arrayexpress
GenomicsPurification of mitochondria from skeletal muscle tissue for transcriptomic analyses reveals localization of nuclear-encoded non-coding RNAs [Mitoplasts]
Purification of mitochondria from skeletal muscle tissue for transcriptomic analyses reveals localization of nuclear-encoded non-coding RNAs [Mitoplasts]
PRJNA1156604 | ENA
GenomicsPurification of mitochondria from skeletal muscle tissue for transcriptomic analyses reveals localization of nuclear-encoded non-coding RNAs [SMvsMT]
Purification of mitochondria from skeletal muscle tissue for transcriptomic analyses reveals localization of nuclear-encoded non-coding RNAs [SMvsMT]
PRJNA1156605 | ENA
Genomics
Meiotic Purification of Dysfunctional Mitochondria in Mouse Oocytes
PRJNA1301552 | ENA
ProteomicsMceC affinity purification and interactome analysis
Anti-FLAG affinty purification of mitochondria isolated from tetracycline inducible HEK293 cells expressing MceC-3XFLAG or control (EV) cells. Equal amounts of mitochondrial protein were solubilised and anti-FLAG immunoprecipitation performed.
ORGANISM(S): Homo sapiens (Human) Coxiella burnetii 
2020-11-12 | PXD019339 | Pride
Effector protein Affinty purification Coxiella 
ProteomicsIdentification of proteins differentially regulated upon perturbed mitochondrial gene expression
The aim of this project was to search for the new factors engaged in mitochondrial nucleic acids metabolism under stress conditions. To this end we treated human 293 cells with mitochondrial replication/transcription inhibitor ethidium bromide and we compared proteomes of untreated vs treated cells....
ORGANISM(S): Homo sapiens (Human) 
2019-06-13 | PXD012055 | Pride
Mitochondria Lc-ms/ms 
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