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The presence of two active X chromosomes (XaXa) is a hallmark of the ground state of pluripotency specific to murine embryonic stem cells (ESCs). Human ESCs invariably exhibit signs of X chromosome inactivation (XCI) and are considered developmentally more advanced than their murine counterparts. W...
ORGANISM(S): Homo sapiens 
The demethylating drug 5-aza-2’-deoxycytidine (5-aza-2dC) is frequently used to investigate the; effect of global DNA demethylation on gene expression in cultured mammalian cells. Here, we utilize a novel method that uses the reactivation of an X-inactivated GFP transgene as a marker to enrich for...
ORGANISM(S): Mus musculus 
Primary mouse dermal fibroblasts from heterozygous female X chromosome-linked GFP mice were sorted through FACS for GFP non-expressing cells (~50% of cells due to X-inactivation). The GFP- cells were treated with 4uM 5-aza-2'-deoxycytidine (5A2dC) for 48 hours with recovery in normal medium for 24 ...
ORGANISM(S): Mus musculus 
Epinephelus fuscoguttatus x Epinephelus lanceolatus Genome sequencing
Epinephelus fuscoguttatus x Epinephelus lanceolatus hap1 genome assembly
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