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TranscriptomicsiCLIP of TDP-43
A stable HEK293 FlpIn T-Rex cells expressing TDP-43 with an N-terminal eGFP-tag was generated that allowed inducible physiological expression of the protein (Ling et al. 2010). Duplicate iCLIP experiments were performed using an antibody targeting eGFP (Abcam ab290). Crosslinked RNA-protein complexe...
ORGANISM(S): Homo sapiens 
2017-05-01 | E-MTAB-4733 | biostudies-arrayexpress
Genomicsena-DATASET-UKBEC-12-03-2015-16:21:20:556-32 - samples
Brain samples for this dataset were provided by the Medical Research Council Sudden Death Brain and Tissue Bank (Edinburgh, UK). All four individuals sampled were of European descent, neurologically normal during life and confirmed to be neuropathologically normal by a consultant neuropathologist...
EGAD00001001274 | EGA
GenomicsRecursive splicing in long vertebrate genes
Recent studies have shown that expression of many genes with exceptionally long introns in the mammalian brain can be perturbed by regulatory factors linked to neurodevelopmental or neurodegenerative disorders1-3, suggesting unique regulatory mechanisms. Here we identify functional recursive splice ...
EGAS00001001170 | EGA
GenomicsThis DAC is the list of persons that, on behalf of the UKBEC consortium, manage the access to the UKBEC data.
Data Access Committee EGAC00001000314
EGAC00001000314 | EGA
TranscriptomicsAlternative splicing in Mouse E18 brain
Total RNA was extracted from wild-type and FUS -/- mouse E18 brain samples using RNeasy kit, cDNA was synthesized using GeneChip WT cDNA Synthesis and Amplification kit (Affymetrix 900673) and hybridised to Affymetrix mouse high-resolution AltSplice microarrays.
ORGANISM(S): Mus musculus 
2013-07-30 | E-MTAB-1229 | biostudies-arrayexpress
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