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ProteomicsGlycoproteomes of naive and LPS stimulated human monocytes and THP-1 cells
CD14+ Monocytes from healthy volunteers were purified by MACS (negative selection) and FACSorting and either left untreated or stimulated for 24h and 48h with LPS. THP-1 cells were stimulated for 4h, 24h and 48h with LPS. Glycoproteins were captured with hydrazide chemistry and tryptic and PNGase F-...
ORGANISM(S): Homo sapiens (Human) 
2017-04-24 | PXD004034 | Pride
Monocytes glycoproteomics 
ProteomicsIdentification of novel glycoproteins expressed on human macrophage surface using glycoproteomics
Prostate cancer is a leading cause of cancer-related deaths of men in the U.S. While localized disease is highly treatable by surgical resection and radiation, cancer that has metastasized remains incurable. Immune cells that primarily scavenge debris, promote prostate cancer angiogenesis and woun...
ORGANISM(S): Homo sapiens (Human) 
2017-03-31 | PXD005080 | Pride
Macrophage glycoproteome Glycoproteomics Prostate cancer Human 
ProteomicsParallel Reaction Monitoring to Assess High-Affinity N-Glycoforms of the Human Fcγ Receptor IIIa (CD16a)
Fc γ-receptor IIIa (FcγRIIIa, or CD16a) is a receptor for the Fc region of IgG antibodies and plays an essential role in the regulation of antibody effector functions. While the activation of CD16a plays a role in a healthy immune response, dysregulated CD16a activity may contribute to tissue damage...
ORGANISM(S): Homo sapiens (Human) 
2026-04-27 | PXD071784 | Pride
Glycosylation Cd16a Oligomannose Igg N162 Immunotherapy High mannose n-linked glycans Fcγriiia Parallel reaction monitoring Monocytes Fc γ (gamma) receptor iiia 
UnknownThe Identification of Macrophage-enriched Glycoproteins Using Glycoproteomics.
Not available
S-EPMC5461535 | biostudies-literature
ProteomicsThermal Proteome Prodiling and Glycoproteomics of U937 cells
Protein glycosylation regulates essential cellular processes including protein folding, stability, and cell-cell interactions; however, how aberrant glycosylation impacts protein function and interaction networks remains poorly understood. Here we combine mass spectrometry-based proteomics, chemical...
ORGANISM(S): Homo sapiens (Human) 
2025-12-11 | PXD071666 | Pride
Human monocytes with perturbed secretory pathway 
UnknownHEXB Drives Raised Paucimannosylation in Colorectal Cancer and Stratifies Patient Risk.
Not available
S-EPMC11932691 | biostudies-literature
UnknownTumor-expressed GPNMB orchestrates Siglec-9<sup>+</sup> TAM polarization and EMT to promote metastasis in triple-negative breast cancer.
Not available
S-EPMC12435292 | biostudies-literature
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