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We used microarrays to examine changes in gene expression in multiple myeloma cell lines following treatment with arsenic trioxide and darinaparsin Experiment Overall Design: Four multiple myeloma cell lines (U266, MM.1s, KMS11, 8226/S) were treated with either arsenic trioxide (ATO) for 6, 24, or 4...
ORGANISM(S): Homo sapiens 
Neutrophil lysis after phagocytosis is a process potentially important in the pathogenesis of community-associated methicillin-resistant S. aureus (CA-MRSA) infection. The mechanism for this process is not currently known. Therefore, to better understand CA-MRSA virulence we used human oligonucleo...
ORGANISM(S): Homo sapiens 
Previously, it has been demonstrated that formate can be utilized by Saccharomyces cerevisiae as additional energy source using cells grown in a glucose-limited chemostat. Here, we investigated utilization of formaldehyde as co-substrate. Since endogenous formaldehyde dehydrogenase activities were i...
ORGANISM(S): Saccharomyces cerevisiae 
The goal of this experiment was to identify transcripts associated with the S. cerevisiae Upf1 protein. Experiment Overall Design: The RNA population from four independent pairs of Upf1p-TAP and mock affinity selections were analyzed by using Affymetrix Yeast Genome S98 Arrays.
ORGANISM(S): Saccharomyces cerevisiae 
Murine ES cell line AB2.2 was infected for 2 and 4 hours with Salmonella Typhimurium. The percent of infected cells was tested by FACS analysis previous to RNA extraction. Total RNA was extracted from uninfected cells and infected with S. Typhimurium SL1344 at 2 and 4 hours infection. The biotin-lab...
ORGANISM(S): Mus musculus 
Oxidative stress is a harmful condition in a cell, tissue, or organ, caused by an imbalnace between reactive oxygen species and other oxidants and the capacity of antioxidant defense systems to remove them. The budding yeast S. cerevisiae has been the major eukaryotic model for studies of response t...
ORGANISM(S): Saccharomyces cerevisiae 
Staphylococcus aureus is a Gram-positive bacteria frequently isolated from patients with bloodstream infections. Endothelial cells (ECs) play an important role in host cell defence against bacteria, and recent reports have shown that infection of ECs with S. aureus induces a wide range of cytokines ...
ORGANISM(S): Homo sapiens 
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