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ProteomicsPhosphorylation sites that influences the RNA-binding activity of Papi
We conducted UV cross-linking immunoprecipitation (CLIP) with and without phosphatase inhibitor and compared the outcome. The topmost band on the western blots appeared more prominent with the inhibitor. The RNA-binding activity of the band was also stronger with the inhibitor. We isolated the band ...
ORGANISM(S): Bombyx Mori 
2022-02-01 | PXD027395 | JPOST Repository
ProteomicsPapi interacting proteins in mitochondrial fraction of BmN4 cells
We incubated Papi-Flag in BmN4 mitochondrial lysates in the presence of 32P-ATP. Upon incubation, Papi-Flag was 32P-labeled, suggesting the presence of kinases for Papi in the fraction. Papi-Flag was immunoprecipitated after incubation in mitochondrial lysates under ATP-free conditions. The proteins...
ORGANISM(S): Bombyx Mori 
2022-02-01 | PXD027396 | JPOST Repository
ProteomicsLabel-free quantification of Xkr4 interactors
Membrane fractions of XRCC4 KO PLB cells expressing SPOT-Xkr4-FLAG and XRCC4 (WT or R270A) were solubilized by detergent and immunoprecipitated with anti-SPOT nanobody-conjugated beads to precipitate Xkr4 interactors, followed by mass spectrometry.
ORGANISM(S): Homo Sapiens (human) 
2021-01-12 | PXD023536 | JPOST Repository
ProteomicsPRM quantification of XRCC4 and Xkr4 peptides that were immunoprecipitated with SPOT-Xkr4-Flag
Several selected peptides of human XRCC4 and mouse Xkr4 were measured by PRM. LC-MS/MS analysis was performed on an EASY-nLC 1200 UHPLC connected to a Q Exactive Plus mass spectrometer through a nanoelectrospray ion source (Thermo Fisher Scientific). Targeted MS/MS scans were acquired by a time-sche...
ORGANISM(S): Homo Sapiens (human) 
2021-01-12 | PXD023537 | JPOST Repository
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