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OfMasc-GFP derivatives or GFP were transiently expressed in OfTC cells. At 2 days post transfection, OfTC cells were treated with 100 nM Bortezomib for 1 day. The OfT1C cells were then fixed with 0.1% formaldehyde, and the fixation was quenched with 300 mM glycine. The cells were lysed on ice in 1 m...
ORGANISM(S): Cellular Organisms 
Spider mites are a group of arachnids belonging in the family Tetranychidae, known to produce nano-scale silk fibers characterized by high Young’s modulus. Silk fibroin gene of spider mites has been computationally suggested through genomic analysis of Tetranychus urticae, but it is yet to be confir...
ORGANISM(S): Tetranychus Kanzawai Tetranychus Urticae Panonychus Citri 

The precise mechanism by which butyrate-producing bacteria in the gut contribute to resistance to respiratory viral infections remains to be elucidated. Here, we describe a gut-lung axis mechanism and report that orally administered Clostridium butyricum (CB) enhances influenza virus infection re...

2023-05-30 | MTBLS4590 | MetaboLights
Identification of the proteins from tryptic digests of Thermosulfidibacter takaii strain ABI70S6 whole-cell proteome followed by nanoLC/OrbitrapFusion MS analysis.
ORGANISM(S): Cellular Organisms 
The selection of signature peptides is critical for reliable peptide quantification. The purpose of this study was to comprehensively assess the digestion efficiency and stability of tryptic peptides and to identify optimal signature peptides for human hepatic transporters and membrane marker protei...
ORGANISM(S): Homo Sapiens (human) 
Proteome analysis of reeled spider silk and web in captive environments
ORGANISM(S): Cyclosa Laticauda Argiope Amoena Yaginumia Sia Dictyna Felis Cyclosa Octotuberculata Parasteatoda Tepidariorum Badumna Insignis Argiope Bruennichi Lycosa Suzukii Trichonephila Clavata Tetragnatha Praedonia 
The purpose of the present study was to validate the enrichment of transporter proteins in plasma membrane fraction prepared from mouse liver and kidney with Membrane Protein Extraction Kit by means of beads homogenize for efficient subcellular fractionation. The crude and plasma membrane fractions ...
ORGANISM(S): Mus Musculus (mouse) 
This dataset includes a proteomic analysis of the ZP protein isolated from MII oocytes in young- and middle-aged mice.
ORGANISM(S): Mus Musculus (mouse) 
Absolute quantification of metabolic enzymes (core metabolic set) was carried out by MRM with internal standard recombinant proteins. Sample digests were labeled with mTRAQdelta0 and internal standard digests were labeled with mTRAQdelta4.
ORGANISM(S): Homo Sapiens (human) 
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