Dataset Information

Proteomic evidence for the silk fibroin genes of spider mites (order Trombidiformes: family Tetranychidae)

ABSTRACT: Spider mites are a group of arachnids belonging in the family Tetranychidae, known to produce nano-scale silk fibers characterized by high Young’s modulus. Silk fibroin gene of spider mites has been computationally suggested through genomic analysis of Tetranychus urticae, but it is yet to be confirmed by proteomic evidences. In this work, we sequenced and assembled the transcriptome from two genera of spider mites, Tetranychus kanzawai and Panonychus citri, and combined with the silk proteomics of T. urticae and P. citri to characterize the fibroin genes through comparative genomics and multi-omics analysis. As a result, two fibroins were identified, that are different genes from the previous computational prediction. Amino acid composition and secondary structure suggests similarity to aciniform or cylindrical spidroins of spider silk, which partly mirrors their mechanical property exhibiting high Young’s modulus. Availability of full-length fibroin sequences of spider mites facilitates the study of the evolution of silk genes that emerged in multiple lineages sometimes in convergent manner, and in the industrial application of artificial protein fibers through the study of the amino acid sequence and the resulting mechanical properties of these silks.

ORGANISM(S): Tetranychus Kanzawai Tetranychus Urticae Panonychus Citri

SUBMITTER: Kazuharu Arakawa

PROVIDER: PXD023661 | JPOST Repository | Wed Mar 24 00:00:00 GMT 2021

REPOSITORIES: jPOST

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Dataset's files

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			Action	DRS
	190627mm_001_Kanzawahadani_%23KO1_001.RAW	Raw
	190627mm_001_Kanzawahadani_%23KO1_001.mgf	Mgf
	190627mm_002_Kanzawahadani_%23KO2_001.RAW	Raw
	190627mm_002_Kanzawahadani_%23KO2_001.mgf	Mgf
	190627mm_003_Mikanhadani_%23MO1_001.RAW	Raw

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Publications

Proteomic evidence for the silk fibroin genes of spider mites (Order Trombidiformes: Family Tetranychidae).

Arakawa Kazuharu K Mori Masaru M Kono Nobuaki N Suzuki Takeshi T Gotoh Tetsuo T Shimano Satoshi S

Journal of proteomics 20210320

Spider mites are a group of arachnids belonging to Acari (mites and ticks), family Tetranychidae, known to produce nanoscale silk fibers characterized by a high Young's modulus. The silk fibroin gene of spider mites has been computationally predicted through genomic analysis of Tetranychus urticae Koch, but it has yet to be confirmed by proteomic evidence. In this work, we sequenced and assembled the transcriptome from two genera of spider mites, Tetranychus kanzawai Kishida and Panonychus citri ...[more]

PMID: 33757880

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