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ProteomicsPhosphorylation sites that influences the RNA-binding activity of Papi
We conducted UV cross-linking immunoprecipitation (CLIP) with and without phosphatase inhibitor and compared the outcome. The topmost band on the western blots appeared more prominent with the inhibitor. The RNA-binding activity of the band was also stronger with the inhibitor. We isolated the band ...
ORGANISM(S): Bombyx Mori 
2022-02-01 | PXD027395 | JPOST Repository
ProteomicsPapi interacting proteins in mitochondrial fraction of BmN4 cells
We incubated Papi-Flag in BmN4 mitochondrial lysates in the presence of 32P-ATP. Upon incubation, Papi-Flag was 32P-labeled, suggesting the presence of kinases for Papi in the fraction. Papi-Flag was immunoprecipitated after incubation in mitochondrial lysates under ATP-free conditions. The proteins...
ORGANISM(S): Bombyx Mori 
2022-02-01 | PXD027396 | JPOST Repository
ProteomicsDIA analysis of FLAG-RPS19 immunoprecipitates in HCT116 cells
Three independent biological replicates of HCT116 cells transiently transfected with FLAG-tagged RPS19 expression vectors or empty vectors were fixed with 0.1% formaldehyde methanol-free (Pierce) and then lysed on ice in 1 ml of HEPES-RIPA2 buffer (20 mM HEPES-NaOH pH7.5, 1 mM EGTA, 1 mM MgCl2, 150 ...
ORGANISM(S): Homo Sapiens (human) 
2026-03-09 | PXD075369 | JPOST Repository
ProteomicsDDA analysis of FLAG-RPS7, RPS17, and RPS19 immunoprecipitates in HCT116 cells
HCT116 cells transiently transfected with FLAG-tagged RPS7, RPS17, or RPS19 expression vectors or empty vectors were fixed with 0.1% formaldehyde methanol-free (Pierce) and then lysed on ice in 1 ml of HEPES-RIPA2 buffer (20 mM HEPES-NaOH pH7.5, 1 mM EGTA, 1 mM MgCl2, 150 mM NaCl, 0.25% Na-deoxychol...
ORGANISM(S): Homo Sapiens (human) 
2026-03-09 | PXD075368 | JPOST Repository
UnknownOseltamivir-resistant influenza viruses A (H1N1) during 2007-2009 influenza seasons, Japan.
Not available
S-EPMC3086245 | biostudies-literature
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