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ProteomicsThe Whole cell lysate and Crude membrane of HEK293T cells
We perform the experiment in order to compare identified membrane proteins from whole cell lysate and membrane fraction in HEK293T cell. The isolation of membrane fractions is critical to obtain a more comprehensive membrane proteome, especially with proteins containing transmembrane domains.
ORGANISM(S): Homo Sapiens (human) 
2022-04-28 | PXD027472 | JPOST Repository
ProteomicsMembrane proteome of renal BBMVs from the mouse model
In order to demonstrate the efficacy of the membrane enrichment method by Urea Wash in the animal model. Kidneys were dissected from 8 weeks old male mice of inbred strain C57BL/6J and flash-frozen in liquid nitrogen followed by storage at -80 °C. Brush border membrane vesicles (BBMV) were prepared...
ORGANISM(S): Mus Musculus (mouse) 
2022-04-28 | PXD027474 | JPOST Repository
ProteomicsMembrane proteome of HEK293T cells
We developed a simple and effective method for “in-depth” membrane proteomics. We optimize the sample preparation for the maximum numbers of the identified membrane proteins using a human cell line, HEK293T, as a model. From this study, we demystified the merits of Urea wash methods for membrane enr...
ORGANISM(S): Homo Sapiens (human) 
2022-04-28 | PXD027473 | JPOST Repository
UnknownSafety and Prognostic Implications of Mechanical Circulatory Support-Assisted Transcatheter Edge-to-Edge Repair: The OCEAN-Mitral Registry.
Not available
S-EPMC12826929 | biostudies-literature
UnknownReal-World Outcomes of the Initial Clip Selection for Transcatheter Edge-to-Edge Repair Using the MitraClip G4 From the Optimized Catheter Valvular Intervention-Mitral Registry.
Not available
S-EPMC12553428 | biostudies-literature
UnknownAssociation Between Direct Oral Anticoagulant Score and Bleeding Events in Patients With Atrial Fibrillation Following Transcatheter Aortic Valve Replacement: A Retrospective Multicenter Cohort Study.
Not available
S-EPMC11681409 | biostudies-literature
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