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ProteomicsmTPP of temperature sensitive proteasome mutants
Temperature sensitive (TS) missense mutants have been foundational in the characterization essential gene function. However, an unbiased approach for the analysis of the biochemical and biophysical changes in TS missense mutants within the context of their functional proteomes is lacking. We applied...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-11-03 | PXD017222 | Pride
Tpp Yeast Proteasome Lc-ms/ms 
ProteomicsFACT, PAF-C, and CKII
Protein-protein interaction analysis for RNA Polymerase II and its elongation factors including: the PAF Complex, the FACT Complex, casein kinase II, DSIF, and TFIIF. These studies also characterized a number of casein kinase II phosphorylation sites on the subunits of the PAF Complex.
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2015-07-20 | MSV000079197 | MassIVE
ProteomicsProteomic Analyses of Plasma from Patients with Fracture Related Infection Reveals Systemic Activation of the Complement and Coagulation Cascades
Patients/Participants: Twenty-seven patients meeting confirmatory FRI criteria were matched to 27 controls based on fracture region, age, and time after surgery Intervention: Tandem Mass Tag (TMT) LC-MS analysis of patient plasma samples Main Outcome Measurements: Abundances for over 1000 proteins w...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-10-15 | MSV000093116 | MassIVE
ProteomicsConsequences of loss of spinophilin on striatal mGluR5 phosphorylation and interactome
Constitutive knockout of the obsessive-compulsive disorder-associated protein, SAPAP3, results in repetitive motor dysfunction, such as excessive grooming, caused by increased mGluR5 activity in striatal medium spiny neurons (MSNs). However, signaling mechanisms that mediate mGluR5-dependent groomin...
ORGANISM(S): Mus musculus (Mouse) 
2022-12-14 | PXD034053 | Pride
Phosphatase targeting proteins Striatum Mouse Tmt-lc/ms 
ProteomicsCharacterization of the redox proteome of Drosophila melanogaster S2 cells and w1118 fly eyes.
Reactive oxygen species (ROS) serve as intracellular signaling molecules; however, in excess ROS molecules are damaging to biomacromolecules such as DNA, lipids, and proteins. The excess accumulation of ROS leads to oxidative stress, disrupting redox homeostasis in the cell and has been linked with ...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-04-27 | PXD034050 | Pride
Drosophila melanogaster schneider s2 cells Drosophila eye Redox proteome Blue light 
ProteomicsGlobal proteomics proteasome mutants
Temperature sensitive(TS)missense mutants have been foundational in the characterization of the function of essential genes due to their pronounced cellular phenotypes that are likely associated with protein functional disruption. However, an unbiased approach for the analysis of the biochemical and...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-11-03 | PXD017223 | Pride
Yeast Tmt Proteasome Tpp 
ProteomicsAffinity Purification Trigger with mTPP
We have applied a BASIL-like approach to increase the signal of low abundance peptides to the study of protein complexes. To accomplish this, we incorporated an affinity purified cleavage and polyadenylation factor (CPF) complex as a “trigger” into our mTPP workflow at a trigger sample to lowest hea...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-09-09 | PXD020689 | Pride
Ap-ms Tpp Lc-ms/ms 
ProteomicsCharacterization of BioID tagging systems in budding yeast and exploring the interactome of the Ccr4-Not complex
The modified E. coli biotin ligase BirA* was the first developed for proximity labeling of proteins (BioID). However, it has low activity at temperatures below 37 C, which reduces its effectiveness in organisms growing at lower temperatures, such as budding yeast. Multiple derivatives of the enzymes...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2024-05-02 | MSV000094663 | MassIVE
ProteomicsCryo-EM Structures of Prion protein filaments from Gerstmann-Sträussler-Scheinker disease
Aggregation of the prion protein (PrP) and formation of PrP amyloid (APrP) is severe in some Prion diseases. In the dominantly inherited prion protein amyloidosis known as Gerstmann-Sträussler-Scheinker (GSS) disease, plaques made of PrP amyloid are a distinct feature. The TTC to TCC mutation in Pr...
ORGANISM(S): Homo sapiens (Human) 
2022-07-18 | PXD032402 | Pride
Cryo-em structures of prion protein filaments from gerstmann-sträussler-scheinker disease 
ProteomicsSuccinyl-CoA synthetase deficiency in mouse forebrain results in hyper-succinylation with perturbed neuronal transcriptional regulation and metabolism
Lysine-succinylation is a subtype of protein acylation associated with metabolic regulation of succinyl-CoA in the TCA cycle. Deficiency of succinyl-CoA synthetase (SCS), the TCA-cycle enzyme catalyzing the reversible conversion of succinyl-CoA to succinate, leads to mitochondrial encephalomyopathy ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-06-21 | MSV000089697 | MassIVE
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