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We performed the analysis of native peptides pool of gametophores, protonema and protoplast cells of the moss. To reduce endogenous proteolytic activity we used an acid extraction buffer with a mixture of plant protease inhibitors. All the actions were performed on ice. After the proper sample prepa...
ORGANISM(S): Physcomitrella patens 
2016-05-18 | PXD000227 | Pride
Short (<100 codons) open reading frames (sORFs) are common features of all genomes. Proof of translation of sORFs is an important step towards finding of functional sORF-encoded peptides (SEPs). We verified translation of our predicted sORFs using mass-spectrometry (MS) analysis which is often cons...
ORGANISM(S): Physcomitrella patens subsp. patens (Moss) 
2019-08-08 | PXD007923 | Pride
Short (<100 codons) open reading frames (sORFs) are common features of all genomes. Proof of translation of sORFs is an important step towards finding of functional sORF-encoded peptides (SEPs). We verified translation of our predicted sORFs using mass-spectrometry (MS) analysis which is often cons...
ORGANISM(S): Physcomitrella patens subsp. patens (Moss) 
2019-08-08 | PXD007922 | Pride
Bryophytes are the most basal of the extant land plants. A major feature of these plants is the biphasic alteration of generations between a dominant haploid gametophyte and a minor diploid sporophyte phase. To analyse the differences in the transcriptome of the early gametophyte (protonema) and ear...
ORGANISM(S): Physcomitrella patens 
Experiment to identify genes for responding to water stress in the moss Physcomitrella patens using various available water stresses such as mannitol, NaCl, and drought. Treatments were carried out on duplicate tissue samples. RNA isolated independently from each tissue sample and monitored for stre...
ORGANISM(S): Physcomitrella patens 
Physcomitrella patens is a model plant representing the group of mosses, which diverged from vascular plant ancestors early after land colonization. To investigate the unique characteristic of the composition and post-translational regulation of Physcomitrella patens photosynthetic apparatus, we hav...
ORGANISM(S): Physcomitrella patens subsp. patens (Moss) 
2019-05-08 | PXD012501 | Pride
The moss Physcomitrella patens is remarkable for the ease with which mutant alleles of any gene can be generated by highly efficient homologous recombination-mediated gene targeting. Targeted transgene integration is believed to be mediated through the capture of transforming DNA by the homologous r...
ORGANISM(S): Physcomitrella patens 
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