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TranscriptomicsMIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing
This SuperSeries is composed of the following subset Series: GSE32180: MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [microarray] GSE32184: MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [deep sequencing] Refer to...
ORGANISM(S): Mus musculus 
2011-11-30 | E-GEOD-32183 | biostudies-arrayexpress
TranscriptomicsMIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [deep sequencing]
Here we show that MIWI is a small RNA-guided ribonuclease (Slicer) that requires extensive complementarity for target cleavage in vitro. Disruption of its catalytic activity in mice by a single point mutation results in male infertility and displays increased accumulation of LINE1 transposon transcr...
ORGANISM(S): Mus musculus 
2011-11-30 | E-GEOD-32184 | biostudies-arrayexpress
TranscriptomicsMIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [microarray]
Here we show that MIWI is a small RNA-guided ribonuclease (Slicer) that requires extensive complementarity for target cleavage in vitro. Disruption of its catalytic activity in mice by a single point mutation results in male infertility and displays increased accumulation of LINE1 transposon transcr...
ORGANISM(S): Mus musculus 
2011-11-30 | E-GEOD-32180 | biostudies-arrayexpress
ProteomicsTEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing.
The PIWI protein MIWI2 and its associated PIWI-interacting RNAs (piRNAs) instruct DNA methylation of young active transposable elements (TEs) in the male germline. Here we show that MIWI2 associates with TEX15 in foetal gonocytes. TEX15 is predominantly a nuclear protein that is not required for piR...
ORGANISM(S): Mus musculus (Mouse) 
2020-06-18 | PXD019087 | Pride
Mouse transposon silencing 
TranscriptomicsA role for Fkbp6 and the chaperone machinery in piRNA amplification and transposon silencing
Epigenetic silencing of transposons by Piwi-interacting RNAs (piRNAs) constitutes an RNA-based genome defense mechanism. Piwi endonuclease action amplifies the piRNA pool by generating new piRNAs from target transcripts by a poorly understood mechanism. Here, we identified mouse Fkbp6 as a factor in...
ORGANISM(S): Bombyx mori 
2012-09-04 | E-GEOD-39203 | biostudies-arrayexpress
ProteomicsEvidence for an RNAi-independent role of DICER-LIKE2 in conferring growth inhibition and basal antiviral resistance
Higher plants encode four or five DICER-LIKE (DCL) enzymes responsible for the production of small non-coding RNAs which function in RNA interference (RNAi). Different RNAi pathways in plants effect transposon silencing, antiviral defense and endogenous gene regulation. DCL2 acts genetically redunda...
ORGANISM(S): Arabidopsis Thaliana 
2024-07-26 | PXD046163 | panorama
TranscriptomicspiRNA-guided slicing of transposon transcripts enforces their transcriptional silencing via specifying the nuclear piRNA repertoire
PIWI-clade Argonaute proteins silence transposon expression in animal gonads. Their target specificity is defined by bound ~23-30nt piRNAs that are processed from single-stranded precursor transcripts via two distinct pathways. Primary piRNAs are defined by the endo-nuclease Zucchini, while biogenes...
ORGANISM(S): Drosophila melanogaster 
2015-08-31 | E-GEOD-71775 | biostudies-arrayexpress
TranscriptomicsWRKY6 Transcription Factor Restricts Arsenate Uptake and Transposon Activation in Arabidopsis
Stress constantly challenges plant adaptation to the environment. Of all stress types, arsenic was a major threat during the early evolution of plants. The most prevalent chemical form of arsenic is arsenate, whose similarity to phosphate renders it easily incorporated into cells via the phosphate...
ORGANISM(S): Arabidopsis thaliana 
2014-04-25 | E-GEOD-49037 | biostudies-arrayexpress
TranscriptomicsMIWI and piRNA-mediated cleavage of messenger RNAs in mouse testes
The piRNA machinery is known for its role in mediating epigenetic silencing of transposons. Recent studies suggest that this function also involves piRNA-guided cleavage of transposon-derived transcripts. As many piRNAs also appear to have the capacity to target diverse mRNAs, this raises the intrig...
ORGANISM(S): Mus musculus 
2015-04-09 | E-GEOD-67683 | biostudies-arrayexpress
TranscriptomicsA SWI/SNF nucleosome remodeling complex acts in non-coding RNA-mediated transcriptional silencing
RNA-mediated transcriptional silencing prevents deleterious effects of transposon activity and controls the expression of protein-coding genes. It involves long non-coding RNAs (lncRNAs)1, which in Arabidopsis thaliana are produced by a specialized RNA Polymerase V (Pol V)2. lncRNAs guide Argonaute-...
ORGANISM(S): Arabidopsis thaliana 
2012-12-01 | E-GEOD-38400 | biostudies-arrayexpress
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