Genome-wide occupancy of PPARbeta/delta in human myofibroblast (WPMY-1 celline) was studied with ChIP-Seq. Additionally, H3K4 trimethylation and RNA polymerase II status was examined.
The secretomes of tumor cells, and macrophages from high grade serous ovarian carcinoma from the ascites of patients undergoing primary surgery were analysed after 24h culture (ex vivo) via obitrap.
MDA-MB-231luc1 cells were treated for 6h with PPARbd specific agonists, antagonists and control substances. In addition the influence of PPARbd on gene expression was analyzed via siRNA mediated PPARbd depletion.
Tumor cells, macrophages and T cells from high grade serous ovarian carcinoma from the ascites of patients undergoing primary surgery were analysed without culturing (ex vivo) via obitrap.
Primary mouse macrophages from wt and PPARD-/- mice, elucidated with thioglycollate, were exposed to PPARbeta/delta specific ligands and the resulting changes in global gene expression were measured.
