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TranscriptomicsTranscription profiling of mouse 2C5/7 myoD-/-;myf5-/- fibroblast cells expressing MyoD, Myf5, myogenin, or hrGFP
Gene expression changes induced by MyoD or Myf5 were examined in a double-knockout fibroblast cell line lacking endogenous functional myoD or myf5 genes. Use of this cell line precluded the possibility of auto- or cross-activation of endogenous myoD or myf5. Myogenin or hrGFP were expressed in paral...
ORGANISM(S): Mus musculus 
2008-06-12 | E-GEOD-3245 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse embryoic fibroblasts expressing wild type MyoD or its non-acetylatable version MyoD RRR to investigate how MyoD acetylation may contribute to differential gene activation.
The transcription factor MyoD can coax na?e fibroblasts or otherwise committed cells to adopt the skeletal muscle phenotype by activating the muscle gene expression program. Activation of muscle gene expression occurs in quantal steps with not all the target genes of MyoD being activated at the same...
ORGANISM(S): Mus musculus 
2007-11-15 | E-GEOD-6487 | biostudies-arrayexpress
TranscriptomicsEnhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by Fusion to a Potent Transactivation Domain
Synthetic transcription factors can be applied to many areas of biotechnology, medicine, and basic research.  Currently, the most common method for engineering synthetic transcription factors has been based on programmable DNA-binding domains of zinc finger proteins, Transcription Activator-Like Ef...
ORGANISM(S): Homo sapiens 
2014-12-11 | E-GEOD-62448 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse muscle satellite cell-derived myoblasts (MyoD-/-, p53-/-)
Muscle satellite cells are a self-renewing pool of stem cells that give rise to daughter myogenic precursor cells in adult skeletal muscle. Published and preliminary data indicated that MyoD and p53 genes are involved in satellite cell differentiation. We would like to know what downstream genes of ...
ORGANISM(S): Mus musculus 
2008-06-12 | E-GEOD-3244 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse embryonic fibroblasts isolated from Myod-/-Myf-/- mice were tranduced with either MDER or MDER+Myog, MDER was activated by the addition of beta-estradiol and samples collected at multiple time points
We used a combination of genome-wide and promoter-specific DNA binding and expression analyses to assess the functional roles of Myod and Myog in regulating the program of skeletal muscle gene expression. Our findings indicate that Myod and Myog have distinct regulatory roles at a similar set of tar...
ORGANISM(S): Mus musculus 
2007-12-07 | E-GEOD-3858 | biostudies-arrayexpress
TranscriptomicsInducible expression of MyoD directly mediates myogenic conversion of human induced pluripotent stem cells (iPSCs) derived from Duchenne muscular dystrophy (DMD).
Genome-wide gene expression analysis of MyoD-infected DMD-specific iPSCs (GM05112-M5.1) on days 0 (untreated), day 3 and day 8 post Dox treatment, human primary myoblasts (undifferentiated and as differentiated myotubes), and undifferentiated iPSCs from healthy donors (iPSCs-1 and iPSCs-2). DMD-spec...
ORGANISM(S): Homo sapiens 
2014-01-09 | E-GEOD-46633 | biostudies-arrayexpress
GenomicsComparison of Genome-Wide Binding of MyoD in Normal Human Myogenic Cells and Rhabdomyosarcomas Identifies Regional and Local Suppression of Promyogenic Transcription Factors (expression profiling).
Rhabdomyosarcoma is a pediatric tumor of skeletal muscle that expresses the myogenic basic helix-loop-helix protein MyoD but fails to undergo terminal differentiation. Prior work has determined that DNA binding by MyoD occurs in the tumor cells, but myogenic targets fail to activate. Using MyoD chro...
ORGANISM(S): Homo sapiens 
2013-08-29 | E-GEOD-50413 | biostudies-arrayexpress
GenomicsConversion of MyoD to a neurogenic factor: binding site specificity determines lineage [ChIP-seq]
MyoD and NeuroD2 are master regulators of myogenesis and neurogenesis and bind to a "shared" E-box sequence (CAGCTG) and a "private" sequence (CAGGTG or CAGATG, respectively). To determine whether private-site recognition is sufficient to confer lineage-specification, we generate...
ORGANISM(S): Mus musculus 
2015-07-31 | E-GEOD-64626 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human rhabdomyosarcoma cells expressing Myod and E-protein heterodimer and controls
Rhabdomyosarcomas (RMS) are characterized by expression of myogenic specification genes, such as MyoD and/or Myf5, as well as their bHLH partners for heterodimerization, the E-proteins. We have shown that expression of a forced heterodimer of MyoD with one of the E2A proteins, E12, leads to differe...
ORGANISM(S): Homo sapiens 
2009-02-21 | E-GEOD-14825 | biostudies-arrayexpress
GenomicsGenome-wide maps of MyoD, MLL4 and histone modifications during MyoD-induced myogenesis in WT and MLL4-/- brown preadipocytes
Enhancers play a central role in cell-type-specific gene expression and are marked by H3K4me1/2. Active enhancers are further marked by H3K27ac. However, the methyltransferases responsible for the deposition of H3K4me1/2 on enhancers remain elusive. Furthermore, the functions of these methyltransfer...
ORGANISM(S): Mus musculus 
2013-12-17 | E-GEOD-50465 | biostudies-arrayexpress
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