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TranscriptomicsTranscription profiling of Arabidopsis roots from slim1 and the parental line grown +/-S condition
Plants utilize soil sulfate for production of sulfur-containing amino acids that serve as essential dietary sulfur sources for animals. Despite the global nutritional significance of this fundamental metabolic process in nature, transcription factors regulating the plant sulfur assimilation pathways...
ORGANISM(S): Arabidopsis thaliana 
2008-06-13 | E-GEOD-4455 | biostudies-arrayexpress
ProteomicsTMT-based proteomic analysis of Atg5-/- mouse brain
The brains of neonatal mice (Atg5+/+ in triplicate, Atg5-/- in quadruplicate, and Atg5-/-;NSE-Atg5 in triplicate) were lysed in 500 µL of 6 M guanidine-HCl containing 100 mM Tris-HCl, pH8.0, and 2 mM DTT. The lysates were dissolved by heating and sonication, followed by centrifugation at 20,000 g fo...
ORGANISM(S): Mus Musculus (mouse) 
2024-06-01 | PXD049220 | JPOST Repository
UnknownYIPF3 and YIPF4 regulate autophagic turnover of the Golgi apparatus
Not available
S-SCDT-10_1038-S44318-024-00131-3 | biostudies-other
UnknownMicroautophagy regulated by STK38 and GABARAPs is essential to repair lysosomes and prevent aging
Not available
S-SCDT-10_15252-EMBR_202357300 | biostudies-other
ProteomicsTMTpro-18plex analysis of siSTK38 or siLuc HeLa cells treated with EBSS or LLOMe
HeLa cells were transfected with STK38 siRNA or Luc siRNA and then treated with EBSS or LLOMe in three biological replicates. The cells were lysed in 500 µL guanidine buffer (6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, 40 mM chloroacetamide). After heating and sonication, proteins (330...
ORGANISM(S): Homo Sapiens (human) 
2023-11-21 | PXD039312 | JPOST Repository
ProteomicssJIA serum proteome profiling with DIA-MS
We performed a DIA-MS proteomic analysis of sera from systemic juvenile idiopathic arthritis with different activity phases using a high protein depletion process. We profiled the proteins in the sera that differed significantly in their activity phase.
ORGANISM(S): Homo Sapiens (human) 
2022-04-26 | PXD030413 | JPOST Repository
ProteomicsProteomics of necrotic granulomas in lungs of C3HeB/FeJ mice infected with M. tuberculosis
C3heB/FeJ mice were infected with M. tuberculosis to form necrotic granulomatous lesions. FFPE samples of infected lungs with granulomas were microdissected into three distinct regions, Caseum, foamy macrophage, and Cell. Proteins were extracted from microdissected samples, followed by LC-MS/MS.
ORGANISM(S): Mus Musculus (mouse) 
2022-09-06 | PXD030088 | JPOST Repository
UnknownJMJD3-mediated senescence is required to overcome stress-induced hematopoietic defects
Not available
S-SCDT-10_1038-S44319-025-00502-9 | biostudies-other
ProteomicsLFQ of GFP-Trap IP-MS from MEF cells expressing GFP-Rubicon
Control or GFP-Rubicon expressing MEFs were treated with or without 0.5uM Apilimod for one hour, and then cross-linked with 0.1% formaldehyde for ten mins at room temperature. After cross-linking was quenched with 100mM glycine for four mins at room temperature, cells were washed with HEPES saline c...
ORGANISM(S): Mus Musculus (mouse) 
2024-06-25 | PXD043695 | JPOST Repository
ProteomicsLFQ of GFP-Trap IP-MS from MEF cells expressing GFP-WIPI2d
Control or GFP-WIPI2d expressing MEFs were treated with or without 0.5uM Apilimod for one hour, and then cross-linked with 0.1% formaldehyde for ten mins at room temperature. After cross-linking was quenched with 100mM glycine for four mins at room temperature, cells were washed with HEPES saline co...
ORGANISM(S): Mus Musculus (mouse) 
2024-06-25 | PXD043701 | JPOST Repository
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