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TranscriptomicsMamld1 knockdown with siRNA in mouse Leydig tumor cells
MAMLD1 (mastermind-like domain containing 1, alias CXorf6) on human chromosome Xq28 is a causative gene for hypospadias, a mild form of 46,XY disorders of sex development. To date, multiple mutations have been identified in patients with various types of hypospadias. In this regard, the mouse homolo...
ORGANISM(S): Mus musculus 
2014-01-26 | E-GEOD-26913 | biostudies-arrayexpress
ProteomicsA stress-reduced passaging technique improves the viability of human pluripotent cells
Xeno-free culture systems have expanded the application of human pluripotent stem cells (PSCs). Here we describe an improved method for the subculture of human PSCs. The revised method significantly facilitated the viability of human PSCs by lowering DNA damage and apoptosis, resulting in more effic...
ORGANISM(S): Homo Sapiens (human) 
2022-01-23 | PXD028731 | JPOST Repository
ProteomicsMulti-omics Characterization of Neutrophil Extracellular Trap Formation in Severe and Mild COVID-19 Infections
Analysis of human plasma from patients either infected or not infected with COVID-19
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-04-28 | MSV000089330 | MassIVE
ProteomicsPost-transcriptionally regulated genes are essential for pluripotent stem cell survival
Here, by combining transcriptome and proteome profiling, we identified 228 post-transcriptionally regulated genes with strict regulation at the protein level in PSCs. Among them, 84 genes were found necessary for the survival of several cell types including PSCs, and 20 genes were identified as spec...
ORGANISM(S): Homo Sapiens (human) 
2022-05-10 | PXD028489 | JPOST Repository
ProteomicsProximity proteomics of T-tubules using miniTurbo-fused LUBEL
To identify candidate genes unbiasedly, we employed biotinylation-based proximity proteomics of T-tubules in vivo. We generated a transgenic line carrying an LUBEL construct fused with miniTurbo (mnTb). We expressed either LUBEL-mnTb or mnTb alone in muscles, and larvae were reared in the presence o...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-11-22 | PXD062634 | JPOST Repository
ProteomicsCo-IP and MS analysis of LacZ or 3xHA:LUBEL-N
HA-LUBEL 1-872 or LacZ (control) was expressed under the control of Mef2-GAL4, and the progenies were raised at 25°C. The 3IL carcass fillet lysates were subjected to anti-HA immunoprecipitation. To identify potential binding partners, we performed immunoprecipitation-mass spectrometry (IP-MS) of LU...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-11-22 | PXD062558 | JPOST Repository
ProteomicsProximity proteomics of T-tubules
To identify candidate genes unbiasedly, we employed biotinylation-based proximity proteomics of T-tubules in vivo. We generated a transgenic line carrying an Amph construct fused with miniTurbo (mnTb). We expressed either Amph-mnTb or mnTb alone in muscles, and larvae were reared in the presence or ...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-11-22 | PXD062543 | JPOST Repository
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