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MetabolomicsHigh-performance nano-flow liquid chromatography column combined with high- and low-collision energy data-independent acquisition enables targeted and discovery identification of modified ribonucleotides by mass spectrometry (Data-independent acquisition)

Over 170 post-transcriptional RNA modifications have been described and are common in all kingdoms of life. These modifications range from methylation to complex chemical structures, with methylation being the most abundant. RNA modifications play a key role in RNA folding and function and their ...

2023-10-26 | MTBLS2959 | MetaboLights
MetabolomicsHigh-performance nano-flow liquid chromatography column combined with high- and low-collision energy data-independent acquisition enables targeted and discovery identification of modified ribonucleotides by mass spectrometry (Mixed-mode column chromatography)

Over 170 post-transcriptional RNA modifications have been described and are common in all kingdoms of life. These modifications range from methylation to complex chemical structures, with methylation being the most abundant. RNA modifications play a key role in RNA folding and function and their ...

2023-10-26 | MTBLS2497 | MetaboLights
MetabolomicsCE-MS Metabolomic and LC-MS Proteomic Analyses of Breast Cancer Exosomes Reveal Alterations in Purine and Carnitine Metabolism

A nano sheath-flow capillary electrophoresis mass spectrometry (CE-MS) system with electrospray ionization was used to profile cationic metabolite cargo in exosomes secreted by non-tumorigenic MCF-10A and tumorigenic MDA-MB-231 breast epithelial cells. CE-MS-based metabolomics revealed increased ...

2024-11-14 | MTBLS11190 | MetaboLights
ProteomicsComparision of Nano and Micro-flow LC-MS/MS Based Single Shot Proteomics
In this study, we systematically optimized the micro-flow LC-MS/MS system based ssDDA proteomics. Our results show that combination of 2 – 3 h gradient with prosit rescore method identified the highest number of proteins and peptides. There is no benefit to increase column length. A single 3 h run i...
ORGANISM(S): Homo sapiens (Human) 
2021-07-01 | PXD024169 | Pride
Single shot proteomics Nano-flow lc Micro-flow lc 
ProteomicsImproved Sensitivity in Low-Input Proteomics using Micro-Pillar Array-based Chromatography
Capitalizing on the massive increase in sample concentrations which are produced by extremely low elution volumes, nano-LC-ESI-MS/MS is currently the most sensitive analytical technology for the comprehensive characterization of complex protein samples. However, despite tremendous technological impr...
ORGANISM(S): Homo sapiens (Human) 
2019-10-23 | PXD014124 | Pride
Nano-lc-esi-ms/ms nano-flow hplc hela cell digest 
UnknownSystematic Investigation of LC Miniaturization to Increase Sensitivity in Wide-Target LC-MS-Based Trace Bioanalysis of Small Molecules.
Not available
S-EPMC9340153 | biostudies-literature
ProteomicsDevelopment and performance evaluation of an ultra-low flow nano liquid chromatography-tandem mass spectrometry set-up
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is the most commonly used technique for the identification and characterization of proteins. The ionization and transmission efficiency of the electrospray process is a critical factor in LC-MS/MS, ultimately limiting the sensitivity of the a...
ORGANISM(S): Homo sapiens (Human) 
2014-07-10 | PXD000396 | Pride
Lc-ms hplc gradient human hela 
UnknownRobust, reproducible and quantitative analysis of thousands of proteomes by micro-flow LC-MS/MS.
Not available
S-EPMC6952431 | biostudies-literature
ProteomicsRT alignment test of DeepRTAlign using data under two gradients
Nano-flow LC-MS/MS analysis of 293T digest in two gradients.
ORGANISM(S): Homo Sapiens 
2023-08-01 | PXD044221 |
UnknownDevelopment of a microfluidic confocal fluorescence detection system for the hyphenation of nano-LC to on-line biochemical assays.
Not available
S-EPMC2990015 | biostudies-literature
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