Nano-lc-ms/ms - OmicsDI

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Proteomics (6562)
Unknown (454)
Metabolomics (14)
Genomics (13)
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Other (7)

Organisms

Schizosaccharomyces pombe (6414)
Saccharomyces cerevisiae (6414)
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Homo sapiens (39)
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cellular organisms (2)
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Methylomirabilis oxygeniifera (SeqCode) (1)
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Organisms

Homo sapiens (4)
Methylomirabilis oxygeniifera (SeqCode) (1)
Xanthomonas campestris (1)
Human adenovirus 7 (1)
Aspergillus fumigatus (1)
Danio rerio (1)
Solanum tuberosum (1)
Mus musculus (1)

Repository

pride (6416)
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Tissue

Cell culture (1365)
Epithelial cell (421)
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Permanent cell line cell (239)
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Blood plasma (183)
Plant cell (174)
Blood (161)
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Cell suspension culture (103)
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Blood cell (97)
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Hepatocyte (66)
Embryonic stem cell (65)
Root (60)
Stem cell (55)
Testis (52)
Urine (49)
Plasma Non-glyco (1)

Disease

Disease free (155)
Breast cancer (145)
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Acute leukemia (62)
Cardiovascular system disease (47)
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Malignant neoplasm of ovary (37)
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Lung cancer (23)
Plasmodium falciparum malaria (21)
Melanoma (21)
Amyotrophic lateral sclerosis (21)
Pancreatic cancer (20)
Non-small cell lung carcinoma (19)
Hepatocellular carcinoma (16)
Chronic myeloid leukemia (14)
Lung adenocarcinoma (13)
Multiple myeloma (13)
Pancreatic ductal adenocarcinoma (13)
Not Available (4)

Technology Type

Mass Spectrometry (6416)
Shotgun proteomics (3539)
Bottom-up proteomics (1121)
Data-dependent acquisition (717)
Affinity purification coupled with mass spectrometry proteomics (714)
Gel-based experiment (456)
Data-independent acquisition (324)
Top-down proteomics (225)
SWATH MS (185)
Chemical cross-linking coupled with mass spectrometry proteomics (148)
SRM/MRM (73)
All-ion fragmentation (46)
DiaPASEF (28)
MSE (26)
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HDMSE (19)
Mass spectrometry imaging (13)
Immunopeptidomics (12)
Mass spectrometry (10)
Metaproteomics (10)
Proteogenomics (10)
Glycoproteomics (5)
Gel-free quantification method (3)
RNA mass spectrometry (3)
Mass spectrometry assay (2)
Gel-based quantification method (1)

Instrument Platform

Q Exactive (9)
LTQ (7)
LTQ Orbitrap Elite (6)
Q Exactive HF (6)
Orbitrap Fusion Lumos (6)
Orbitrap Fusion (5)
LTQ Orbitrap Velos (5)
Instrument (4)
LTQ Orbitrap (4)
Q Exactive HF-X (3)
Easy n-LC and LTQ Orbitrap XL mass spectrometers (both from Thermo Fisher, San Jose, CA, USA) (2)
Nano-electrospray source, were used for nano LC-MS/MS (2)
LTQ Orbitrap XL ETD (2)
MaXis II (2)
Impact (2)
LTQ XL (2)
QExactive (2)
Orbitrap Eclipse (2)
Q Exactive Plus (2)
Orbitrap Exploris 480 (2)
Nano-LC/MS/MS (1)
Q Exactive plus (Thermo Scientific instrument model) (1)
Southern University of Science and Technology (1)
Waters Synapt G2 HDMS (1)
Bruker Impact II (1)
Easy n-LC and LTQ Orbitrap XL mass spectrometers (both from Thermo Fisher, San Jose, CA, USA), each equipped with a nano-electrospray source, were used for nano LC-MS/MS. (1)
MS:1000031 (1)
QSTAR XL (1)
LTQ XL ETD (1)
6520A Quadrupole Time-of-Flight LC/MS (1)

Publication Date

2025 (896)
2022 (787)
2023 (782)
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2026 (748)
2021 (664)
2020 (491)
2019 (415)
2018 (327)
2017 (247)
2016 (224)
2015 (108)
2014 (78)
2013 (21)
2010 (6)
2012 (3)
2011 (2)
2009 (1)
2006 (1)

UNIPROT ID/AC

GAG2A_HUMAN (1)
ISL2_HUMAN (1)
FBCD1_HUMAN (1)
B4DXG7_HUMAN (1)
GG12C_HUMAN (1)
PRA12_HUMAN (1)
MCAF2_HUMAN (1)
FGF18_HUMAN (1)
MSH5_HUMAN (1)
ISL1_HUMAN (1)
LY6K_HUMAN (1)
9606.ENSP00000384214 (1)
CTAG2_HUMAN (1)
PAGE1_HUMAN (1)
PLPL3_HUMAN (1)
CDN2B_HUMAN (1)
GAGE1_HUMAN (1)
HIF1A_HUMAN (1)
O4A15_HUMAN (1)
UBP37_HUMAN (1)
9606.ENSP00000478568 (1)
GAG13_HUMAN (1)
GG12J_HUMAN (1)
9606.ENSP00000416249 (1)
TR64C_HUMAN (1)
GAGE7_HUMAN (1)
S15A5_HUMAN (1)
AGRE3_HUMAN (1)
CENPM_HUMAN (1)
TRI61_HUMAN (1)

ENSEMBL ID

ENSMUSP00000141097 (2)
ENSMUSG00000072849 (1)
ENSG00000170477 (1)
ENSMUSG00000098557 (1)

CHEBI ID

CHEBI:16335 (5)
CHEBI:16750 (4)
CHEBI:21891 (4)
CHEBI:16704 (4)
CHEBI:178090 (3)
CHEBI:20129 (3)
CHEBI:19229 (3)
CHEBI:19228 (3)
CHEBI:20794 (3)
CHEBI:19227 (3)
CHEBI:19062 (3)
CHEBI:69426 (3)
CHEBI:20607 (3)
CHEBI:17802 (3)
CHEBI:17562 (3)
CHEBI:19065 (3)
CHEBI:16020 (3)
CHEBI:17596 (3)
CHEBI:87239 (2)
CHEBI:167041 (2)
CHEBI:85682 (2)
CHEBI:134236 (2)
CHEBI:140361 (2)
CHEBI:140360 (2)
CHEBI:134459 (2)
CHEBI:134237 (2)
CHEBI:134435 (2)
CHEBI:141289 (2)
CHEBI:138335 (2)
CHEBI:85707 (2)

Metabolite Name

Adenosine (4)
2'-O-methyluridine (3)
7-methylguanosine (3)
3-methylcytidine (3)
5-methylcytidine (3)
2'-O-methylguanosine (3)
2'-O-methylcytidine (3)
1-methylinosine (3)
1-methyladenosine (3)
2'-O-methyladenosine (3)
1-methylguanosine (3)
4-methylcytidine (2)
2-methylguanosine (2)
2'-O-methylinosine (2)
6-methyladenosine (2)
8-methyladenosine (2)
2-methyladenosine (2)
3-methyluridine (2)
5-methyluridine (2)
3-methylpseudouridine (2)
1-methylpseudouridine (2)
Guanosine (2)
N6-methyladenosine (2)
Cytidine (2)
Guanosine (2)
Uridine (2)
Pseudouridine (2)
Inosine (2)
DAG 44:1 (1)
DAG 42:2 (1)

First Public Date

2024 (4)
2022 (3)
2025 (1)
2023 (1)
2021 (1)
2020 (1)
2014 (1)
2013 (1)

Classification

fungi (1)
viruses (1)

Modification

15.994915 (20)
4-tetrahydrothiazine-3-carboxylic acid (19)
4x(15)N labeled L-arginine (17)
2x(15)N labeled L-lysine (15)
57.021464 (15)
42.010565 (10)
-17.026549 (9)
Not available (8)
+3amu) (6)
79.966331 (4)
-18.010565 (4)
4 (4)
0.984016 (3)
N6-dimethyl-L-lysine (3)
56.026215 (2)
4x(2)H labeled dimethylated residue (2)
58.005479 (2)
N6 (2)
57.02146 (2)
0.984 (2)
15.99491 (2)
32.056407 (1)
Heavy form - site K (1)
Heavy form (1)
Medium form - site K (1)
Medium form - site N-term (1)
45.029395 (1)
59.036279 (1)
43.00581 (1)
4'-dihydroxyphenylalanine (1)

Study type

Transcription profiling by array (3)
RNA-seq of coding RNA (1)

Release Date

2021 (51)
2020 (41)
2019 (39)
2022 (34)
2014 (33)
2025 (31)
2018 (29)
2016 (25)
2024 (24)
2023 (24)
2017 (22)
2015 (21)
2010 (17)
2012 (16)
2011 (14)
2013 (13)
2009 (12)
2007 (4)
2008 (3)
2026 (2)
2006 (1)
2004 (1)
2000 (1)

Lab affiliation

University of Vienna, Faculty of Chemistry, Department of Analytical Chemistry (74)
Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02215, USA., Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA (28)
Department of Biochemistry and Functional Proteomics, Institute of Biology II, Faculty of Biology and BIOSS Centre for Biological Signalling Studies, University of Freiburg, 79104 Freiburg im Breisgau, Germany (17)
Ludwig-Maximilians-Universität München Genzentrum Laboratory for Functional Genome Analysis Feodor-Lynen-Str. 25 D-81377 München (14)
Centre for Genomic Regulation (13)
Head of Proteomics (13)
Department of Convergence Medicine, Convergence Medicine Research Center/Biomedical Research Center, Asan Medical Center, Asan Institute for Life Sciences, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea. (12)
Chair of Biochemistry II, Theodor Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany (12)
TIGEM (12)
Head of the Curie Institute Mass Spectrometry Platform (11)
Max-Planck-Institute for Biology of Ageing Department of Mitochondrial Proteostasis Joseph-Stelzmann-Str. 9b 50931 Cologne (10)
NCI/NIH CSB (10)
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences (9)
Centre for Proteome Research, University of Liverpool, Liverpool, UK (9)
Department of Ecology & Evolutionary Biology, University of California, Los Angeles, CA (8)
Head of Laboratory of Neuro-Oncology, Neurology, Clinical & Cancer Proteomics (8)
Max Planck Institute for Terrestrial Microbiology Karl-von-Frisch Str. 10 35043 Marburg Germany (8)
University of Warwick (8)
University of Edinburgh (8)
Proteomics core facility, Biomedical Research Institute, Seoul National University Hospital (8)
Evolutionary and translational venomics Lab, Instituto de Biomedicina de Valencia, CSIC (7)
Center for Reproductive Evolution, Biology Department, Syracuse University (7)
Biomolecular characterization unit, Center for Sustainable Resource Science, RIKEN (7)
Department of Biotechnology, TU Delft, The Netherlands (7)
NARO Institute of Crop Science (6)
LMU Munich Plant Biochemistry and Physiology, Germany (6)
Department of Biomedical Sciences, University of Padova, Italy (6)
Biozentrum, University of Basel, Switzerland (6)
Department of Biotechnology, Delft University of Technology, The Netherlands. (6)
Department of Biology, IISER Pune (6)

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Performance comparisons of nano-LC systems, electrospray sources and LC-MS-MS platforms.

Not available

S-EPMC3886563 | biostudies-literature

Optimization of label-free nano LC-MS/MS analysis of the placental proteome

We optimized a protocol to analyze the placental proteome by means of label-free nano-LC-MS/MS mass spectrometry with regard to sample treatment, protein extraction, and protein digestion, in order to obtain a high protein concentration for identification of a specific protein signature according to...

ORGANISM(S): Homo sapiens (Human)

2020-10-13 | PXD020438 | Pride