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Immunoprecipitation experiments using anti-RFFL antibody. Samples were loaded in technical duplicates in EvoTips, and chromatographic separation was done on an in-house column and the 30 samples per day (SPD) method on Evosep One system (Evosep). Peptides were detected and fragmented using timsTOF P...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-01-16 | MSV000096868 | MassIVE
A549 cells were washed with ice-cold PBS three times and harvested using mechanical scraping. Cell pellets were processed as described previously (Marathe et al., 2021). Briefly, the pellet was re-solubilized in Urea-Thiourea Buffer (6M Urea, 2M Thio-urea in Tris-Cl) containing protease inhibitor co...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-01-15 | MSV000096861 | MassIVE
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