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ProteomicsProteomic analysis of bone marrow-derived macrophages of mice treated with recombinant Ssa1, a protein enriched in the hypoxic secretome of Candida albicans.
Bone marrow-derived macrophages from mice were treated with recombinant Ssa1, a protein enriched in the hypoxic secretome of Candida albicans.
ORGANISM(S): Mus Musculus (mouse) 
2024-01-29 | PXD046766 | JPOST Repository
ProteomicsProteome analysis of secretory substances containing proteins, extracellular vehicles, and others secreted from Candida albicans cultured under normal and hypoxic conditions.
Candida albicans were cultured under normal and hypoxic conditions and collected secretome from the culture supernatant with centrifugation. The secretome contains secretory proteins, extracellular vehicles, and others.
ORGANISM(S): Candida Albicans 
2024-01-29 | PXD046763 | JPOST Repository
ProteomicsThe proteins identified from Staphylococcus aureus-derived extracellular vesicles (SaEVs)
Staphylococcus aureus strain ATCC 1718 was inoculated at 0.5% in 400 mL of Brain Heart Infusion broth for 8 h under aerobic conditions. The supernatant was collected by centrifugation twice at 5,000 x g, 4°C for 20 min, filtrated through a 0.45 µm filter to remove all remaining bacterial cells. S. a...
ORGANISM(S): Staphylococcus Aureus 
2024-01-30 | PXD043854 | JPOST Repository
ProteomicsThe quantitative proteomic analysis of Pseudomonas aeruginosa treated with or without Staphylococcus aureus-derived extracellular vesicles (SaEVs)
Pseudomonas aeruginosa was prepared and adjusted to an initial bacterial number of OD600 = 1.0 in Tryptic soy broth with or without 5 μg/mL SaEVs. After incubation at 37°C for 24 h, the bacterial cells were collected by centrifugation at 1,100 x g, 4°C for 15 min, followed by washing twice with phos...
ORGANISM(S): Pseudomonas Aeruginosa 
2024-01-30 | PXD043857 | JPOST Repository
ProteomicsQuantitative proteomic analysis of extracellular membrane vesicles from Acinetobacter baumannii (AbOMVs) isolated under normoxic and hypoxic conditions.
Acinetobacter baumannii were cultured in Brain Heart Infusion medium (BD Bioscience). For normoxic condition, the cultures were shaken at 125 rpm for 24 h, whereas for hypoxic condition, the cultures were placed under static condition for 48 h. Outer membrane vesicles from A. baumannii (AbOMVs) in t...
ORGANISM(S): Acinetobacter Baumannii 
2023-03-16 | PXD037014 | JPOST Repository
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