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ProteomicsNative holdup (nHU) to measure binding affinities from cell extracts
Characterizing macromolecular interactions is essential for understanding cellular processes, yet nearly all methods used to detect protein interactions from cells are qualitative. Here, we introduce a native holdup (nHU) approach that requires minimal sample preparation, investigates binding mechan...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD034790 | Pride
Protein interaction Mass spectrometry Native holdup 
ProteomicsNative holdup (nHU) to measure binding affinities from cell extracts
Characterizing macromolecular interactions is essential for understanding cellular processes, yet nearly all methods used to detect protein interactions from cells are qualitative. Here, we introduce the native holdup (nHU) approach to quantify equilibrium binding constants and explore binding mecha...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD036024 | Pride
Mass spectrometry Protein-protein interaction Affinity constant determination 
UnknownNative holdup (nHU) to measure binding affinities from cell extracts.
Not available
S-EPMC9770967 | biostudies-literature
ProteomicsIdentification and quantitative characterization of protein-DNA interactions with the native holdup assay: GATA1 regulation of the plasma membrane calcium pump
Polymorphisms in regulatory regions of the genome can alter RNA and protein expression and are implicated in a wide range of diseases. It has been recently discovered that a common haplotype in the erythroid-specific promoter of the ATP2B4 gene alters the expression of Plasma Membrane Calcium ATPase...
ORGANISM(S): Homo sapiens (Human) 
2026-09-14 | PXD055181 | Pride
Protein-dna interaction Gata1 Native holdup 
ProteomicsAffinity-ranking BIN1 interactions and screening natural BIN1 variants that may cause neuromuscular disorders
Deletion of the protein-protein interaction SH3 domain of the membrane remodeling BIN1 protein was found to lead to centronuclear myopathy in patients, yet only few interaction partners of BIN1 SH3 have been identified so far. Here we used the holdup assay to proteome-wide measure steady-state affin...
ORGANISM(S): Homo sapiens (Human) 
2024-07-11 | PXD040169 | Pride
Bin1 interactions Native holdup 
UnknownThe non-catalytic DNA polymerase ε subunit is an NPF motif recognition protein.
Not available
S-EPMC12808100 | biostudies-literature
ProteomicsThe non-catalytic epsilon DNA polymerase subunit is an NPF motif recognition protein
Short linear motifs (SLiMs) in disordered protein regions direct numerous protein–protein interactions, yet most remain uncharacterized. The Asn-Pro-Phe (NPF) motif is a well-known EH-domain ligand implicated in endocytosis, but here we reveal that the non-catalytic subunit of human DNA polymerase e...
ORGANISM(S): Homo Sapiens 
2025-03-13 | MSV000097307 | MassIVE
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