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ProteomicsDependency of NELF-E-KAT2B epigenetic axis in breast cancer carcinogenesis
Cancer cells undergo transcriptional reprogramming to drive tumor progression and metastasis. Here, we identified the transcriptional complex, NELF (Negative elongation factor), as an important regulator of this process. Using cancer cell lines and patient-derived tumor organoids, we demonstrated th...
ORGANISM(S): Homo Sapiens (human) 
2023-01-28 | PXD031304 | JPOST Repository
ProteomicsEnhancer RNAs stimulate Pol II pause release by harnessing multivalent interactions to NELF
UV-light-induced protein-RNA cross-linking followed by MS analysis was used to identify RNA-binding regions of enhancer RNAs (eRNAs) to the negative elongation factor (NELF) complex, and NELF as part of the paused elongation complex (PEC) of human RNA polymerase II.
ORGANISM(S): Homo sapiens (Human) 
2022-03-08 | PXD030569 | Pride
Paused elongation complex (pec) Protein-rna cross-linking Uv cross-linking Negative elongation factor (nelf) Protein-rna complexes Rna polymerase ii 
GenomicsRegulation of Mammalian Cell Growth and Survival By an RNA Polymerase II-Pausing Factor (ChIP-seq)
Many mammalian genes are occupied by paused RNA polymerase II (pol II) at promoter-proximal regions on both sides of transcription start sites (TSSs). However, the consequences of pol II pausing on gene expression and cell biology are not fully understood. Here we report that genetic ablation of t...
ORGANISM(S): Mus musculus 
2011-08-17 | E-GEOD-24113 | biostudies-arrayexpress
GenomicsIntegrator complex regulates NELF-mediated RNA Polymerase II pause/release and processivity at coding genes [ChIP-seq]
RNAPII pausing/termination shortly after initiation is a hallmark of gene regulation. However, the molecular mechanisms involved are still to be uncovered. Here, we show that NELF interacts with Integrator complex subunits (INTScom) forming a stable complex with RNPII and Spt5. The interaction betwe...
ORGANISM(S): Homo sapiens 
2015-05-19 | E-GEOD-60584 | biostudies-arrayexpress
GenomicsNELF-dependent transcription activation
Jurkat T-cells were latently infected with a defective HIV provirus carrying a GFP reporer gene. The cells were superinfected with a lentiviral vector expressing shRNA to NELF-E. The impact of NELF-depletion on RNAP II distribution on cellular genes and the HIV provirus was measured by ChIP-Seq. D...
ORGANISM(S): Homo sapiens 
2014-03-26 | E-GEOD-47481 | biostudies-arrayexpress
TranscriptomicsIntegrator complex regulates NELF-mediated RNA Polymerase II pause/release and processivity at coding genes [RNA-seq]
RNAPII pausing/termination shortly after initiation is a hallmark of gene regulation. However, the molecular mechanisms involved are still to be uncovered. Here, we show that NELF interacts with Integrator complex subunits (INTScom) forming a stable complex with RNPII and Spt5. The interaction betwe...
ORGANISM(S): Homo sapiens 
2015-05-19 | E-GEOD-60585 | biostudies-arrayexpress
TranscriptomicsGlobal Analysis of the Drosophila NELF complex
To determine the physiological targets of the NELF complex, and provide insight into the mechanism of NELF activity in vivo. Experiment Overall Design: Drosophila melanogaster S2 cells, obtained from the Drosophila Genomics Resource Center, were untreated, or treated with dsRNA for 72 hours, as desc...
ORGANISM(S): Drosophila melanogaster 
2008-10-18 | E-GEOD-6141 | biostudies-arrayexpress
ProteomicsMS-based determination of NELF-A phosphorylation
LC-MS/MS analysis of Flag-NELF-A purified from TPA-stimulated HEK293 cells
ORGANISM(S): Homo Sapiens (human) 
2022-11-15 | PXD038094 | JPOST Repository
GenomicsGenome-wide map of RNA polymerase II, NELF, and DSIF in HeLa cells
Most metazoan promoters have RNA polymerase II (Pol II) paused slightly downstream of the transcription start site by NELF and DSIF. This pausing keeps these promoters available for rapid induction by P-TEFb, whose activity causes NELF to dissociate and Pol II and DSIF to elongate on the gene. ChIP-...
ORGANISM(S): Homo sapiens 
2014-02-01 | E-GEOD-53008 | biostudies-arrayexpress
GenomicsThe negative elongation factor NELF promotes active transcription of Drosophila ecdysone-dependent genes
The negative elongation factor NELF promotes active transcription of Drosophila ecdysone-dependent genes
PRJNA659365 | ENA
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