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This study investigates immune-related proteins in the sea anemone Nematostella vectensis using immunoprecipitation followed by label-free quantitative proteomics. Specific antibodies against the immune-related protein RLRb were used to enrich interacting proteins. The proteomic profiles of specific...
ORGANISM(S): Nematostella vectensis 
2026-08-24 | PXD064383 | Pride
Environmental contamination from heavy metals poses a global concern for the marine environment, as heavy metals are passed up the food chain and persist in the environment long after the pollution source is contained. Cnidarians, dating back to 700 million years ago, play an important role in shapi...
ORGANISM(S): Nematostella vectensis 
NvElav1::mOrange is a stable transgenic line that labels a large fraction of the nervous system of the sea anemone Nematostella vectensis (Nakanishi et al., Development 2012). Two week old primary polyps were dissociated and the NvElav1::mOrange positive cells were enriched by FACS.
ORGANISM(S): Nematostella vectensis 
We performed Immuno precipitation using antibodies against Nematostella vectensis Argonauts proteins (i.e AGO1 and AGO2). Samples of the IP were analyzed by LC-MSMS in order to idetify the argonautes and prove antibodies specifity.
ORGANISM(S): Nematostella vectensis 
2020-10-14 | PXD011644 | Pride
Nematostella vectensis Genome sequencing
Nematostella vectensis Raw sequence reads
NvNcol3::mOrange2 is a stable transgenic line that labels cnidocytes(stinging cells) of the sea anemone Nematostella vectensis (Nakanishi et al., Development 2012). Two week old primary polyps were dissociated and the NvNcol3::mOrange2 positive and negative cells were enriched by FACS.
ORGANISM(S): Nematostella vectensis 
Using the sea anemone Nematostella vectensis as a model for early branching metazoans, we show that CoREST is an animal-specific protein that assembles a conserved histone-modifying complex including Lsd1 and HDAC1/2. We further show that the Nematostella complex is similar in composition to vertebr...
ORGANISM(S): Nematostella vectensis 
2022-08-08 | PXD033068 | Pride
Nematostella vectensis Transcriptome or Gene expression
Long read sequencing of Nematostella vectensis individuals
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