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Newly synthesized RNA analysis in WT, SAGA and ATAC mutant mouse embryonic stem cells
Innate stimulation with TLR ligands leads to the activation of various genes in macrophages and various populations of these cells may exhibit different responses. Here wanted to delineate and characterize these transcriptional responses in newly established, self-renewing, in vitro grown non-trans...
ORGANISM(S): Mus musculus 
Transcriptome-based mapping of m6A sites in newly synthesized mRNA population in U2OS cells.
Effects of m6A modification on turnover of newly synthesized mRNAs at the transcriptome level.
A nuclear export block triggers the decay of newly synthesized polyadenylated RNA
As a newly identified mRNA modification, the regulation of ac4C remains largely unexplored. RNA-binding proteins (RBPs) that specifically binds to ac4C modification and mediate downstream cellular activities (readers) have not been reported yet. We synthesized acetylated and non-acetylated RNA probe...
ORGANISM(S): Homo sapiens (Human) 
2021-07-20 | PXD027014 | Pride
To better understand ferroptosis and cuproptosis, here we systematically and quantitatively analyzed newly synthesized proteins (NSPs), which reflect rapid changes in gene expression during cell death without the interference from the existing proteins. Integrating metabolic labeling with multiplexe...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-11-13 | MSV000099889 | MassIVE
Detection of newly synthesized RNA reveals transcriptional reprogramming during ZGA and a role of Obox3 in totipotency acquisition
To analyse the genome-wide impact of inactivation of the ATAC or SAGA coactivator complexes on RNA polymerase II (Pol II) transcription, we purified newly synthesized RNA from mutant mouse embryonic stem (ES) cell lines in which subunits of ATAC (Yeats2, Zzz3) or SAGA (Supt7l) are inactivated or dep...
ORGANISM(S): Mus musculus 
2021-08-03 | GSE175904 | GEO
A convenient single-cell newly synthesized transcriptome assay reveals FLI1 regulon downregulation during T-cell activation
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