112251 Results

Show all   Save search   Copy query
Sort   by:  
 Page size 
MetabolomicsNext-generation biomonitoring of the early-life chemical exposome in neonatal and infant development

Exposure to synthetic and natural chemicals is a major environmental risk factor in the etiology of many chronic diseases. Investigating complex co-exposures is necessary for a holistic assessment in exposome-wide association studies. In this work, a sensitive liquid chromatography-tandem mass sp...

2023-10-26 | MTBLS2442 | MetaboLights
MetabolomicsA next-generation yeast chassis: comprehensive characterization of a robust fast-growing Saccharomyces cerevisiae

Robust and efficient hosts are necessary to facilitate advances in biotechnology. We unveil Saccharomyces cerevisiae strain XP as a promising new microbial chassis owing to its high growth rate and robustness. This strain has a doubling time of 48.2 min, which is 12.8 min faster than the...

2024-09-26 | MTBLS7328 | MetaboLights
MetabolomicsOpen source discovery of starting points for next generation chemoprotective antimalarial drugs (Biofocus 1)
Not available
ORGANISM(S): Homo sapiens 
ST001074 | MetabolomicsWorkbench
TranscriptomicsMapping translocation breakpoints by next-generation sequencing
Balanced chromosome rearrangements (BCRs) can cause genetic diseases by disrupting or inactivating specific genes, and the characterisation of breakpoints in disease-associated BCRs has been instrumental in the molecular elucidation of a wide variety of genetic disorders. However, mapping chromosome...
ORGANISM(S): Homo sapiens 
2010-05-22 | E-GEOD-10115 | biostudies-arrayexpress
ProteomicsA functional connection between the Microprocessor and a variant NEXT complex
In mammalian cells, primary miRNAs are cleaved at their hairpin structures by the Microprocessor complex, whose core is composed of DROSHA and DGCR8. Here, we show that 5’flanking regions, resulting from Microprocessor cleavage, are targeted by the RNA exosome in mouse embryonic stem cells (mESCs). ...
ORGANISM(S): Mus musculus (Mouse) 
2026-05-22 | PXD053925 | Pride
Zcchc8-dgcr8 interaction Rna structure Microprocessor Nuclear rna decay The next complex 
GenomicsDNA copy number, including telomeres and mitochondria, assayed using next-generation sequencing
DNA copy number variations occur within populations and aberrations can cause disease. We sought to develop an improved lab-automatable, cost-efficient, accurate platform to profile DNA copy number. We developed a sequencing-based assay of nuclear, mitochondrial, and telomeric DNA copy number that ...
ORGANISM(S): Homo sapiens 
2010-04-02 | E-GEOD-21159 | biostudies-arrayexpress
TranscriptomicsDeepCAGE and DeepSAGE with proliferating and differentiated C2C12 mouse myoblasts
We use tag-based next generation sequencing analysis to quantify gene expression levels during myogenic differentiation and focused on the identifcation of novel 3' ends with DeepSAGE and novel 5' ends with DeepCAGE. Illumina DeepCAGE and DeepSAGE with C2C12 cells at time points T0 (proliferating) a...
ORGANISM(S): Mus musculus 
2010-07-06 | E-GEOD-21580 | biostudies-arrayexpress
TranscriptomicsNext Generation Sequencing of Wild-Type FVB/NJ Mouse Cardiac Small RNA, after 1 week pressure overload
Purpose: Next-generation sequencing (NGS) provides for quantitation of RNA abundances and comparison of RNA abundances within tissues and cells in a manner not possible with previous microarray technologies. 5 female mice were subjected to a sham operation, and 5 female mice were subjected to transv...
ORGANISM(S): Mus musculus 
2014-04-18 | E-GEOD-56891 | biostudies-arrayexpress
TranscriptomicsHigh-throughput SuperSAGE for digital gene expression analysis of multiple samples using Next Generation Sequencing
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined “High-Throughput (HT-) SuperSAGE”. SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from expressed transcripts. In the present protocol, index...
ORGANISM(S): Danio rerio 
2010-09-30 | E-GEOD-20682 | biostudies-arrayexpress
TranscriptomicsIncreased sensitivity of next generation sequencing-based expression profiling after globin reduction in human blood RNA
Transcriptome analysis is of great interest in clinical research, where significant differences between individuals can be translated into biomarkers of disease. Although next generation sequencing provides robust, comparable and highly informative expression profiling data, with several million of ...
ORGANISM(S): Homo sapiens 
2012-01-25 | E-GEOD-33701 | biostudies-arrayexpress
Sort   by:  
 Page size