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GenomicsGenome-wide mapping of OCT4, NANOG and CTCF in human embryonic stem cells
Transcription factors and their specific interactions with targets are crucial in specifying gene expression programs. To gain insights into the transcriptional regulatory networks in embryonic stem cells, we used chromatin immunoprecipitation coupled to ultra-high-throughput DNA sequencing (ChIP-se...
ORGANISM(S): Homo sapiens 
2010-03-06 | E-GEOD-20650 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of 3iL human embryonic stem cells
Human embryonic stem cells (hESCs) are isolated from the inner cell mass of the blastocysts. The pluripotent properties of hESCs enable the derivation of cell-types or tissues of different lineages for potential applications such as therapeutics discovery and regenerative medicine. Even though hES...
ORGANISM(S): Homo sapiens 
2014-12-12 | E-MTAB-2031 | biostudies-arrayexpress
TranscriptomicsPRDM14 Knockdown in human embryonic stem cells
To identify the gene changes after PRDM14 knockdown 6 samples were collected in total. Each sample is done in triplicate and samples were collected at 3 days after transfection. Luciferase knockdown serves as negative control and PRDM14 knockdown is the experimental sample.
ORGANISM(S): Homo sapiens 
2010-10-20 | E-GEOD-22795 | biostudies-arrayexpress
GenomicsGenome-wide mapping of PRDM14 binding sites in human embryonic stem cells
PRDM14 belongs to the PR (PRDI-BF1 and RIZ) domain proteins (PRDM) family which is a subclass of the SET domain proteins, a common domain found in histone modifying enzymes. PRDM14 has been previously implicated to regulate self-renewal of hESCs as knock-down of PRDM14 induced expression of differen...
ORGANISM(S): Homo sapiens 
2010-10-20 | E-GEOD-22767 | biostudies-arrayexpress
TranscriptomicsComparison of hiPSCs, hESCs and fibroblasts
In our study, PRDM14 and NFRkB were found to enhance the reprogramming of human fibroblasts to hiPSCs in the presence of OCT4, SOX2, KLF4, with/without c-MYC (OSK/OSKC). To examnie if the obtained hiPSC share similar expression signature with hESC, we conducted the microarray analysis on the hiPSCs,...
ORGANISM(S): Homo sapiens 
2010-10-20 | E-GEOD-22792 | biostudies-arrayexpress
TranscriptomicsMicroRNA profiling of mouse embryonic stem cells during retinoic acid-induced differentiation
Changes in microRNA expression profile during retinoic acid-induced differentiation of mouse embryonic stem cells.
ORGANISM(S): Mus musculus 
2007-04-01 | E-MEXP-977 | biostudies-arrayexpress
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